Overview
- External ontology
- TOGO:M1266 — Modified Cellulolytic Haloarchaea Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M1266 records the solid-agar JCM Medium 1181 preparation of Modified Cellulolytic Haloarchaea Medium with washed agar plus the same MDS, trace-element, phosphate, vitamin, NH4Cl, cellobiose, and Na2CO3 stocks used by the liquid recipe.
Composition
flowchart LR
CultureMech_007798["modified_cellulolytic_haloarchaea_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007798 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Agar["Agar"]
CultureMech_007798 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007798 --> ing2_Yeast_extract__BD_Difco_
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
124.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1181 brings the MDS salt water, trace elements, and yeast extract to 958 ml with distilled water. |
| 2 | Agar | 20.0 G_PER_L |
CHEBI:2509
agar
|
JCM Medium 1181 adds 20 g/L agar for solid medium. |
| 3 | Yeast extract (BD-Difco) | 0.1 G_PER_L | JCM Medium 1181 lists 0.1 g/L Yeast extract (BD-Difco). |
Preparation
- Combine MDS salt water, JCM 1079 Trace element solution, yeast extract, and distilled water.
- Autoclave the base medium before adding sterile NH4Cl, phosphate, vitamin, and cellobiose stocks.
- Readjust pH to 7.0 with sterilized 10% Na2CO3 solution if necessary.
Solutions
MDS salt water
Dose: 833.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 240.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| MgCl2 x 6H2O | 30.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| MgSO4 x 7H2O | 35.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| KCl | 7.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| 1 M CaCl2 solution | 5.0 ML_PER_L | JCM Medium 574 adds 5.0 ml/L 1 M CaCl2 solution to MDS salt water. |
| Distilled water | 1.0 L | JCM Medium 574 prints this component in its stock solution. |
JCM Medium 1181 adds 833.0 ml/L MDS salt water from JCM Medium 574.
Trace element solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA | 5.0 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| FeSO4 x 7H2O | 2.0 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| ZnSO4 x 7H2O | 0.10 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| MnCl2 x 4H2O | 0.03 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| H3BO3 | 0.3 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| CoCl2 x 6H2O | 0.2 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| CuCl2 x 2H2O | 0.01 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| NiCl2 x 6H2O | 0.02 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| Na2MoO4 x 2H2O | 0.03 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 1079 prints this component in its stock solution. |
JCM Medium 1181 adds 1.0 ml/L Trace element solution from JCM Medium 1079.
1 M NH4Cl
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NH4Cl | 1.0 MOLAR | JCM Medium 1181 identifies this as a 1.0 M stock solution. |
JCM Medium 1181 adds 5.0 ml/L 1 M NH4Cl after autoclaving.
Potassium phosphate buffer
Dose: 2.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| 1 M K2HPO4 | 417.0 ML_PER_L | JCM Medium 574 prepares Potassium phosphate buffer from 83.4 volumes of 1 M K2HPO4, 16.6 volumes of 1 M KH2PO4, and an equal volume of distilled water. |
| 1 M KH2PO4 | 83.0 ML_PER_L | JCM Medium 574 prepares Potassium phosphate buffer from 83.4 volumes of 1 M K2HPO4, 16.6 volumes of 1 M KH2PO4, and an equal volume of distilled water. |
| Distilled water | 500.0 ML_PER_L | JCM Medium 574 prints this component in its stock solution. |
JCM Medium 1181 adds 2.0 ml/L Potassium phosphate buffer from JCM Medium 574.
Trace vitamins
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 197 prints this component in its stock solution. |
JCM Medium 1181 adds 5.0 ml/L Trace vitamins from JCM Medium 197.
0.2 M Cellobiose solution
Dose: 30.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Cellobiose | 0.2 MOLAR | JCM Medium 1181 identifies this as a 0.2 M stock solution. |
JCM Medium 1181 adds 30.0 ml/L 0.2 M Cellobiose solution after autoclaving.
10% Na2CO3 solution
Dose: variable ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 10 PERCENT_W_V | JCM Medium 1181 identifies this as a 10% w/v stock solution. |
JCM Medium 1181 readjusts pH to 7.0 with sterilized 10% Na2CO3 solution if necessary.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.251212Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1266 |
| 2026-01-28T19:48:23.855020Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 solution |
| 2026-03-09T08:04:39.435286+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:19:51.550007Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007798 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.977948+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Cellulolytic Haloarchaea Medium" to "modified_cellulolytic_haloarchaea_medium" for consistent matching |
| 2026-05-16T09:03:08.978657+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.896175+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_togo_jcm_archaea_score15.py | RESOLVED_TOGO_JCM_ARCHAEA_SCORE15 | TOGO M1266 records the solid-agar JCM Medium 1181 preparation of Modified Cellulolytic Haloarchaea Medium with washed agar plus the same MDS, trace-element, phosphate, vitamin, NH4Cl, cellobiose, and Na2CO3 stocks used by the liquid recipe. |