Overview
- External ontology
- TOGO:M1267 — Ferrous Iron Yeast Extract Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1267 Original source: JCM - JCM_M1182 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1182
Composition
flowchart LR
CultureMech_007799["ferrous_iron_yeast_extract_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007799 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007799 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_007799 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_KCl["KCl"]
CultureMech_007799 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_007799 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Na2SO4["Na2SO4"]
CultureMech_007799 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_Ca_NO3_2_4H2O["Ca(NO3)2・4H2O"]
CultureMech_007799 --> ing6_Ca_NO3_2_4H2O
CHEBI_86159["CHEBI:86159"]
ing6_Ca_NO3_2_4H2O -.-> CHEBI_86159
ing7_H2SO4["H2SO4"]
CultureMech_007799 --> ing7_H2SO4
CHEBI_26836["CHEBI:26836"]
ing7_H2SO4 -.-> CHEBI_26836
ing8_0_01_M_H2SO4["0.01 M H2SO4"]
CultureMech_007799 --> ing8_0_01_M_H2SO4
ing9_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007799 --> ing9_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_2H2O -.-> CHEBI_75213
ing10_H3BO3["H3BO3"]
CultureMech_007799 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_007799 --> ing11_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_7H2O -.-> CHEBI_32312
ing12_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_007799 --> ing12_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_5H2O -.-> CHEBI_31440
ing13_CoSO4_7H2O["CoSO4・7H2O"]
CultureMech_007799 --> ing13_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_7H2O -.-> CHEBI_91244
ing14_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_007799 --> ing14_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing14_Na2WO4_2H2O -.-> CHEBI_63939
ing15_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_007799 --> ing15_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing15_MnSO4_xH2O -.-> CHEBI_86360
ing16_NiSO4_6H2O["NiSO4・6H2O"]
CultureMech_007799 --> ing16_NiSO4_6H2O
CHEBI_53001["CHEBI:53001"]
ing16_NiSO4_6H2O -.-> CHEBI_53001
ing17_Na2SeO4["Na2SeO4"]
CultureMech_007799 --> ing17_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing17_Na2SeO4 -.-> CHEBI_77775
ing18_NaVO3["NaVO3"]
CultureMech_007799 --> ing18_NaVO3
CHEBI_75221["CHEBI:75221"]
ing18_NaVO3 -.-> CHEBI_75221
ing19_Cr2_SO4_3_xH2O["Cr2(SO4)3・xH2O"]
CultureMech_007799 --> ing19_Cr2_SO4_3_xH2O
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.05 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.45 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.15 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Ca(NO3)2・4H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.01 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 9 | 0.01 M H2SO4 | 1 G_PER_L | Role: Mineral source; Properties: Defined component, Solution, Inorganic compound | |
| 10 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.5 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
10 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoSO4・7H2O
Co.7H2O.O4S 281.101 g/mol
|
1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
1 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiSO4・6H2O
Ni.O4S 154.756 g/mol
|
1 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
1 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NaVO3
Na.O3V 121.931 g/mol
|
0.1 G_PER_L |
CHEBI:75221
sodium metavanadate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | Cr2(SO4)3・xH2O | 0.5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace elements solution (see below)
Dose: 1 G_PER_L
1.0% Yeast extract solution
Dose: 20 G_PER_L
Role: Nutrient source; Properties: Undefined component
1 M FeSO4 solution* (pH 2.0)
Dose: 10 G_PER_L
Role: Mineral source; Properties: Solution, Defined component, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.252627Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1267 |
| 2026-01-28T19:48:15.819571Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:04:26.098143+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:51.559537Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007799 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.987346+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ferrous Iron Yeast Extract Medium" to "ferrous_iron_yeast_extract_medium" for consistent matching |
| 2026-05-16T09:03:31.899201+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:00.231051+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:38.407260+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:43.492237+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:56.816737+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |