chloroflexus_medium

CultureMech:007800 bacterial COMPLEX LIQUID
Original name: Chloroflexus Medium

Overview

External ontology
TOGO:M1268 — Chloroflexus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1268 Original source: JCM - JCM_M1183 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1183

Composition

flowchart LR
    CultureMech_007800["chloroflexus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007800 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007800 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KH2PO4["KH2PO4"]
    CultureMech_007800 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007800 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007800 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_007800 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_HEPES["HEPES"]
    CultureMech_007800 --> ing6_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing6_HEPES -.-> CHEBI_46756
    ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007800 --> ing7_Yeast_extract__BD_Difco_
    ing8_Na2S2O3_5H2O["Na2S2O3・5H2O"]
    CultureMech_007800 --> ing8_Na2S2O3_5H2O
    CHEBI_32150["CHEBI:32150"]
    ing8_Na2S2O3_5H2O -.-> CHEBI_32150
    ing9_Biotin["Biotin"]
    CultureMech_007800 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_007800 --> ing10_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing10_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing11_Thiamine_HCl["Thiamine・HCl"]
    CultureMech_007800 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_Pyridoxine_HCl["Pyridoxine・HCl"]
    CultureMech_007800 --> ing12_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_HCl -.-> CHEBI_30961
    ing13_Folic_acid["Folic acid"]
    CultureMech_007800 --> ing13_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing13_Folic_acid -.-> CHEBI_27470
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_007800 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843
    ing15_Riboflavin["Riboflavin"]
    CultureMech_007800 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_007800 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_007800 --> ing17_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_DL_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
101.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 100.0]
2 NaCl
Cl.Na 58.443 g/mol
0.5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 HEPES 3 G_PER_L CHEBI:46756
HEPES
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
8 Yeast extract (BD-Difco) 1 G_PER_L Properties: Undefined component
9 Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
0.1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
 Properties: Defined component, Simple component, Inorganic compound
10 Biotin
C10H16N2O3S 244.316 g/mol
2 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
11 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
5 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
5 G_PER_L CHEBI:49105
thiamine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13 Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
10 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Folic acid
C19H19N7O6 441.404 g/mol
2 G_PER_L CHEBI:27470
folic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15 Vitamin B12 0.5 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16 Riboflavin
C17H20N4O6 376.369 g/mol
5 G_PER_L CHEBI:17015
riboflavin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
17 Nicotinic acid
C6H5NO2 123.111 g/mol
5 G_PER_L CHEBI:15940
nicotinic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
5 G_PER_L CHEBI:31345
Calcium pantothenate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

NaHCO3 solution

Dose: 10 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

CaCl2・2H2O solution

Dose: 1 G_PER_L

Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

Na2S・9H2O solution

Dose: 1 G_PER_L

Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component

Trace vitamins solution (see below)

Dose: 1 G_PER_L

Trace elements solution (see Medium [M305])

Dose: 1 G_PER_L

Cross-reference to Medium M305

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.255456Z togo-import Imported from TOGO Medium Source: JCM, ID: M1268
2026-01-28T19:48:41.779098Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:10.449785+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T03:09:42.233244Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:51.571383Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007800
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.999425+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Chloroflexus Medium" to "chloroflexus_medium" for consistent matching
2026-05-16T09:03:08.990317+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:31.907498+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:30.164422+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:47.442627+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.