atalassotoga_azc2201_medium

CultureMech:007801 bacterial COMPLEX LIQUID
Original name: Atalassotoga AZC2201 Medium

Overview

External ontology
TOGO:M1269 — Atalassotoga AZC2201 Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1269 Original source: JCM - JCM_M1184 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1184

Composition

flowchart LR
    CultureMech_007801["atalassotoga_azc2201_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007801 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007801 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007801 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007801 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_007801 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_K2HPO4["K2HPO4"]
    CultureMech_007801 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_007801 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007801 --> ing7_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_6H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_007801 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007801 --> ing9__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing9__NH4_2SO4 -.-> CHEBI_62946
    ing10_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
    CultureMech_007801 --> ing10_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing11_N2["N2"]
    CultureMech_007801 --> ing11_N2
    CHEBI_17997["CHEBI:17997"]
    ing11_N2 -.-> CHEBI_17997

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.02 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
6 K2HPO4
HO4P.2K 174.174 g/mol
0.02 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
7 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
8 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 KCl
Cl.K 74.551 g/mol
0.3 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.3 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
11 L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.3 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
12 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Fe--Ni--WO--Se solution (see Medium [M1199])

Dose: 1 G_PER_L

Cross-reference to Medium M1199

1% Yeast extract solution

Dose: 10 G_PER_L

Role: Nutrient source; Properties: Undefined component

1 M Fructose solution

Dose: 20 G_PER_L

Role: Carbon source; Properties: Defined component, Solution, Organic compound

1 M Na2S2O3 solution

Dose: 10 G_PER_L

Properties: Defined component, Inorganic compound, Solution

Vitamin solution (see Medium [M1239])

Dose: 1 G_PER_L

Cross-reference to Medium M1239

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.258291Z togo-import Imported from TOGO Medium Source: JCM, ID: M1269
2026-01-28T19:48:08.300536Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:13.368823+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 7 solution(s) from ingredients
2026-03-13T06:19:51.581951Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007801
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.010689+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Atalassotoga AZC2201 Medium" to "atalassotoga_azc2201_medium" for consistent matching
2026-05-16T09:03:09.002149+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.919846+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.370101+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.448565+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.