lb_luria_bertani_agar_with_5_nacl_ph_9_0

CultureMech:007803 bacterial COMPLEX SOLID_AGAR
Original name: LB (Luria-Bertani) Agar With 5% NaCl (pH 9.0)

Overview

External ontology
TOGO:M1270 — LB (Luria-Bertani) Agar With 5% NaCl (pH 9.0)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1270 Original source: JCM - JCM_M1185 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1185

Composition

flowchart LR
    CultureMech_007803["lb_luria_bertani_agar_with_5_nacl_ph_9_0"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007803 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007803 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Agar["Agar"]
    CultureMech_007803 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007803 --> ing3_Yeast_extract__BD_Difco_
    ing4_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_007803 --> ing4_Tryptone__BD_Difco_

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
4 Yeast extract (BD-Difco) 5 G_PER_L Properties: Undefined component
5 Tryptone (BD-Difco) 10 G_PER_L Properties: Undefined component

Solutions

Na2CO3 solution

Dose: variable VARIABLE

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.263887Z togo-import Imported from TOGO Medium Source: JCM, ID: M1270
2026-01-28T19:48:15.249684Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:04:25.397525+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:51.596357Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007803
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.026464+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB (Luria-Bertani) Agar With 5% NaCl (pH 9.0)" to "lb_luria_bertani_agar_with_5_nacl_ph_9_0" for consistent matching
2026-05-16T09:03:09.016053+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.937451+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.