Overview
- External ontology
- TOGO:M1273 — Marine Chloroflexi Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M1273 points to JCM Medium 1188; the current JCM Medium 1188 formulation contains mineral salts, yeast extract, peptone, referenced trace vitamin/mineral/Se-W solutions, four post-autoclave anaerobic stocks, distilled water, and pH 7.0.
Composition
flowchart LR
CultureMech_007806["marine_chloroflexi_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_007806 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_007806 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_007806 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_007806 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaCl["NaCl"]
CultureMech_007806 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Yeast_extract["Yeast extract"]
CultureMech_007806 --> ing5_Yeast_extract
ing6_Peptone["Peptone"]
CultureMech_007806 --> ing6_Peptone
ing7_Distilled_water["Distilled water"]
CultureMech_007806 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 1188 lists 0.1 g/L. |
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.0 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
JCM Medium 1188 lists 4.0 g/L. |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.0 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
JCM Medium 1188 lists 1.0 g/L. |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 1188 lists 0.5 g/L. |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
JCM Medium 1188 lists 5.0 g/L. |
| 6 | Yeast extract | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
JCM Medium 1188 lists 1.0 g/L. |
| 7 | Peptone | 1.0 G_PER_L | JCM Medium 1188 lists 1.0 g/L. | |
| 8 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1188 lists 1000 ml/L. |
Preparation
- Mix KH2PO4, MgCl2 x 6 H2O, CaCl2 x 2 H2O, NH4Cl, NaCl, yeast extract, peptone, trace stocks, and distilled water.
- Adjust pH to 7.0.
- Bring to a boil and cool under an N2-CO2 (4:1, v/v) gas stream.
- Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers, and autoclave.
- After cooling, aseptically and anaerobically add sodium pyruvate, NaHCO3, L-cysteine, and Na2S stock solutions.
Solutions
Trace vitamins solution
Dose: 2.0 ML_PER_L
JCM Medium 1188 lists 2.0 ml/L Trace vitamins solution from JCM Medium 284.
Trace mineral solution
Dose: 1.0 ML_PER_L
JCM Medium 1188 lists 1.0 ml/L Trace mineral solution from JCM Medium 852.
Se/W solution
Dose: 1.0 ML_PER_L
JCM Medium 1188 lists 1.0 ml/L Se/W solution from JCM Medium 852.
1 M Sodium pyruvate
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium pyruvate | 1.0 MOLAR |
JCM Medium 1188 adds 10.0 ml/L filter-sterilized 1 M Sodium pyruvate.
8% NaHCO3 solution
Dose: 25.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 80.0 G_PER_L |
JCM Medium 1188 adds 25.0 ml/L filter-sterilized 8% NaHCO3 solution.
5% L-Cysteine x HCl x H2O solution
Dose: 6.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| L-Cysteine x HCl x H2O | 50.0 G_PER_L |
JCM Medium 1188 adds 6.0 ml/L 5% L-Cysteine x HCl x H2O solution.
5% Na2S x 9H2O solution
Dose: 6.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 50.0 G_PER_L |
JCM Medium 1188 adds 6.0 ml/L 5% Na2S x 9H2O solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.269061Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1273 |
| 2026-01-28T19:48:17.756902Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: sodium pyruvate |
| 2026-03-09T08:04:29.424327+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:51.618948Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007806 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.050440+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Marine Chloroflexi Medium" to "marine_chloroflexi_medium" for consistent matching |
| 2026-05-16T09:03:40.241474+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:12.884034+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_jcm_1188_score20.py | RESOLVED_JCM_1188_SCORE20_GRAPH | TOGO M1273 points to JCM Medium 1188; the current JCM Medium 1188 formulation contains mineral salts, yeast extract, peptone, referenced trace vitamin/mineral/Se-W solutions, four post-autoclave anaerobic stocks, distilled water, and pH 7.0. |