Overview
- External ontology
- TOGO:M1283 — Saltwater Medium For Oceanic AOA
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1283 Original source: JCM - JCM_M1198 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1198
Composition
flowchart LR
CultureMech_007817["saltwater_medium_for_oceanic_aoa"]
ing0_Distilled_water["Distilled water"]
CultureMech_007817 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007817 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007817 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007817 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007817 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_KBr["KBr"]
CultureMech_007817 --> ing5_KBr
CHEBI_32030["CHEBI:32030"]
ing5_KBr -.-> CHEBI_32030
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
980 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
26 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
KBr
Br.K 119.002 g/mol
|
0.1 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
1 M NaHCO3 solution*
Dose: 2 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1 M NH4Cl solution
Dose: 1 G_PER_L
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Solution
3 mM KH2PO4 solution
Dose: 5 G_PER_L
Role: Buffer; Properties: Solution, Inorganic compound, Defined component
7.5 mM FeNaEDTA solution* (pH 7.0)
Dose: 1 G_PER_L
Properties: Defined component, Organic compound, Solution
HEPES solution (see Medium [M1060])
Dose: 10 G_PER_L
Cross-reference to Medium M1060
Modified trace element mixture (see Medium [M1060])
Dose: 1 G_PER_L
Cross-reference to Medium M1060
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.284311Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1283 |
| 2026-01-28T19:48:33.397361Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:55.374852+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:19:51.702804Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007817 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.136143+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Saltwater Medium For Oceanic AOA" to "saltwater_medium_for_oceanic_aoa" for consistent matching |
| 2026-05-16T09:03:09.108557+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.043599+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.461076+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.490619+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |