Overview
- External ontology
- TOGO:M1285 — ELBE Methanotroph Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1285 Original source: JCM - JCM_M1200 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1200
Composition
flowchart LR
CultureMech_007819["elbe_methanotroph_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007819 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007819 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007819 --> ing2_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_6H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_007819 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaNO3["NaNO3"]
CultureMech_007819 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_2_M_HEPES_buffer__pH_7_0_["2 M HEPES buffer (pH 7.0)"]
CultureMech_007819 --> ing5_2_M_HEPES_buffer__pH_7_0_
ing6_methane["methane"]
CultureMech_007819 --> ing6_methane
CHEBI_16183["CHEBI:16183"]
ing6_methane -.-> CHEBI_16183
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
992 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.12 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.93 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.17 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 6 | 2 M HEPES buffer (pH 7.0) | 5 G_PER_L | Role: Buffer; Properties: Defined component, Solution | |
| 7 |
methane
CH4 16.043 g/mol
|
variable VARIABLE |
CHEBI:16183
methane
|
Properties: Gas |
Solutions
Phosphate buffer (see Medium [M560])
Dose: 0.15 G_PER_L
Cross-reference to Medium M560
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Vitamin solution (see Medium [M560])
Dose: 1 G_PER_L
Cross-reference to Medium M560
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.287609Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1285 |
| 2026-01-28T19:48:08.126311Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: methane |
| 2026-03-09T08:04:13.080140+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:19:51.715315Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007819 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.149314+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ELBE Methanotroph Medium" to "elbe_methanotroph_medium" for consistent matching |
| 2026-05-16T09:03:09.124336+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.059108+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-20T19:01:17.917375+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |