marinitoga_hydrogenitolerans_medium

CultureMech:007830 bacterial COMPLEX LIQUID
Original name: Marinitoga Hydrogenitolerans Medium

Overview

External ontology
TOGO:M1297 — Marinitoga Hydrogenitolerans Medium
Applications
  • Microbial cultivation
pH
6.0
Notes
TOGO M1297 records JCM Medium 1210 with sea salts, MES, yeast extract, tryptone, resazurin, distilled water, and an N2 gas atmosphere. JCM 1210 adjusts the base medium to pH 6.0, bubbles it with N2 for 5 min, distributes it into culture vessels under N2, seals with butyl rubber stoppers, autoclaves, then adds autoclaved glucose, Na2S x 9H2O, and L-cysteine HCl H2O stock solutions aseptically and anaerobically.

Composition

flowchart LR
    CultureMech_007830["marinitoga_hydrogenitolerans_medium"]
    ing0_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_007830 --> ing0_Sea_salts__Sigma_
    ing1_MES["MES"]
    CultureMech_007830 --> ing1_MES
    CHEBI_39005["CHEBI:39005"]
    ing1_MES -.-> CHEBI_39005
    ing2_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
    CultureMech_007830 --> ing2_Yeast_extract__Oxoid_
    ing3_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_007830 --> ing3_Tryptone__BD_Difco_
    ing4_Resazurin["Resazurin"]
    CultureMech_007830 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Distilled_water["Distilled water"]
    CultureMech_007830 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_N2["N2"]
    CultureMech_007830 --> ing6_N2
    CHEBI_17997["CHEBI:17997"]
    ing6_N2 -.-> CHEBI_17997

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Sea salts (Sigma) 30.0 G_PER_L JCM Medium 1210 lists 30.0 g/L Sea salts (Sigma).
2 MES
C6H13NO4S 195.24 g/mol
4.0 G_PER_L CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
 JCM Medium 1210 lists 4.0 g/L MES.
3 Yeast extract (Oxoid) 1.0 G_PER_L JCM Medium 1210 lists 1.0 g/L Yeast extract (Oxoid).
4 Tryptone (BD-Difco) 1.0 G_PER_L JCM Medium 1210 lists 1.0 g/L Tryptone (BD-Difco).
5 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
 JCM Medium 1210 lists 0.5 mg/L Resazurin.
6 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 1210 lists 1.0 L Distilled water.
7 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 JCM Medium 1210 bubbles the medium with N2 for 5 min, distributes the medium into culture vessels under N2, and stores supplement stocks under an N2 gas atmosphere.

Preparation

  1. Mix sea salts, MES, yeast extract, tryptone, resazurin, and distilled water.
  2. Adjust to pH 6.0.
  3. Bubble the medium with N2 for 5 min, distribute under N2, and seal with butyl rubber stoppers.
  4. Autoclave at 121 degrees C for 15 min.
  5. Cool after autoclaving.
  6. Add autoclaved and N2-stored glucose, Na2S x 9H2O, and L-cysteine HCl H2O stock solutions aseptically and anaerobically.

Solutions

1.0 M Glucose solution

Dose: 14.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Glucose 1.0 MOLAR 1.0 M Glucose solution is represented from the stock label as 1.0 M Glucose.
JCM Medium 1210 adds 14.0 ml/L 1.0 M Glucose solution aseptically and anaerobically after autoclaving and cooling.

5% Na2S x 9H2O solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2S x 9H2O 5.0 PERCENT_W_V 5% Na2S x 9H2O solution is represented from the stock label as 5.0 % w/v Na2S x 9H2O.
JCM Medium 1210 adds 10.0 ml/L 5% Na2S x 9H2O solution aseptically and anaerobically after autoclaving and cooling.

5% L-Cysteine HCl H2O solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl H2O 5.0 PERCENT_W_V 5% L-Cysteine HCl H2O solution is represented from the stock label as 5.0 % w/v L-Cysteine HCl H2O.
JCM Medium 1210 adds 10.0 ml/L 5% L-Cysteine HCl H2O solution aseptically and anaerobically after autoclaving and cooling.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.307372Z togo-import Imported from TOGO Medium Source: JCM, ID: M1297
2026-01-28T19:48:18.795935Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:31.182914+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:51.801184Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007830
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.237648+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Marinitoga Hydrogenitolerans Medium" to "marinitoga_hydrogenitolerans_medium" for consistent matching
2026-05-16T09:03:09.205214+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.137908+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1297_score15.py RESOLVED_TOGO_M1297_SCORE15 TOGO M1297 records JCM Medium 1210 with sea salts, MES, yeast extract, tryptone, resazurin, distilled water, and an N2 gas atmosphere. JCM 1210 adjusts the base medium to pH 6.0, bubbles it with N2 for 5 min, distributes it into culture vessels under N2, seals with butyl rubber stoppers, autoclaves, then adds autoclaved glucose, Na2S x 9H2O, and L-cysteine HCl H2O stock solutions aseptically and anaerobically.