modified_makkars_medium_c

CultureMech:007832 bacterial COMPLEX LIQUID
Original name: Modified Makkar's Medium C

Overview

External ontology
TOGO:M1299 — Modified Makkar's Medium C
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1299 Original source: JCM - JCM_M1212 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1212

Composition

flowchart LR
    CultureMech_007832["modified_makkars_medium_c"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007832 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007832 --> ing1_Yeast_extract
    ing2_Resazurin["Resazurin"]
    CultureMech_007832 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_NaHCO3["NaHCO3"]
    CultureMech_007832 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Casitone__BD_Difco_["Casitone (BD-Difco)"]
    CultureMech_007832 --> ing4_Casitone__BD_Difco_
    ing5_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
    CultureMech_007832 --> ing5_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing5_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing6_CO2["CO2"]
    CultureMech_007832 --> ing6_CO2
    CHEBI_16526["CHEBI:16526"]
    ing6_CO2 -.-> CHEBI_16526
    ing7_N2["N2"]
    CultureMech_007832 --> ing7_N2
    CHEBI_17997["CHEBI:17997"]
    ing7_N2 -.-> CHEBI_17997
    ing8_K2HPO4["K2HPO4"]
    CultureMech_007832 --> ing8_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing8_K2HPO4 -.-> CHEBI_131527
    ing9_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007832 --> ing9_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_7H2O -.-> CHEBI_31795
    ing10_NaCl["NaCl"]
    CultureMech_007832 --> ing10_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing10_NaCl -.-> CHEBI_26710
    ing11_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007832 --> ing11_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_2H2O -.-> CHEBI_86158
    ing12_KH2PO4["KH2PO4"]
    CultureMech_007832 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007832 --> ing13__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing13__NH4_2SO4 -.-> CHEBI_62946

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
652.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 650.0, 1.0, 1.0]
2 Yeast extract 2.5 G_PER_L Role: Nutrient source; Properties: Undefined component
3 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
4 NaHCO3
CHO3.Na 84.006 g/mol
6 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 Casitone (BD-Difco) 10 G_PER_L Properties: Undefined component
6 L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
1 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
7 CO2
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
8 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
9 K2HPO4
HO4P.2K 174.174 g/mol
3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
10 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
11 NaCl
Cl.Na 58.443 g/mol
6 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
12 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
13 KH2PO4
H2O4P.K 136.084 g/mol
3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
14 (NH4)2SO4
2H4N.O4S 132.141 g/mol
6 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component

Solutions

Solution I (see below)

Dose: 150 G_PER_L

Solution II (see below)

Dose: 150 G_PER_L

Rumen fluid, clarified (see Medium [M258])

Dose: 50 G_PER_L

Cross-reference to Medium M258

0.2 M Cellobiose solution

Dose: 20 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.312619Z togo-import Imported from TOGO Medium Source: JCM, ID: M1299
2026-01-28T19:48:36.335106Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2; Added default concentration for ingredient: N2
2026-03-09T08:05:00.586492+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:09:32.415279Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:51.824644Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007832
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.260805+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Makkar's Medium C" to "modified_makkars_medium_c" for consistent matching
2026-05-16T09:03:09.232152+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.165698+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.568965+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.526328+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.