Overview
- External ontology
- TOGO:M12 — Bifidobacterium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M12 Original source: JCM - JCM_M19 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=19
Composition
flowchart LR
CultureMech_007834["bifidobacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007834 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_K2HPO4["K2HPO4"]
CultureMech_007834 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Tween_80["Tween 80"]
CultureMech_007834 --> ing2_Tween_80
CHEBI_53426["CHEBI:53426"]
ing2_Tween_80 -.-> CHEBI_53426
ing3_Glucose["Glucose"]
CultureMech_007834 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007834 --> ing4_Yeast_extract__BD_Difco_
ing5_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
CultureMech_007834 --> ing5_Beef_extract__BD_Difco_
ing6_Casein_peptone__tryptic_digest["Casein peptone, tryptic digest"]
CultureMech_007834 --> ing6_Casein_peptone__tryptic_digest
ing7_sodium_ascorbate["sodium ascorbate"]
CultureMech_007834 --> ing7_sodium_ascorbate
CHEBI_113451["CHEBI:113451"]
ing7_sodium_ascorbate -.-> CHEBI_113451
ing8_L__cysteine_HCl["L--cysteine・HCl"]
CultureMech_007834 --> ing8_L__cysteine_HCl
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Simple component, Defined component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 5 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 6 | Beef extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 7 | Casein peptone, tryptic digest | 10 G_PER_L | Properties: Undefined component | |
| 8 |
sodium ascorbate
C6H7O6.Na 198.106 g/mol
|
variable VARIABLE |
CHEBI:113451
sodium ascorbate
|
Properties: Defined component, Organic compound, Solution |
| 9 | L--cysteine・HCl | variable VARIABLE |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.111252Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M12 |
| 2026-01-28T19:48:42.536055Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: sodium ascorbate; Added default concentration for ingredient: L--cysteine・HCl |
| 2026-03-13T06:19:51.835499Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007834 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.272290+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Bifidobacterium Medium" to "bifidobacterium_medium" for consistent matching |
| 2026-05-16T09:03:09.243100+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.179282+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.533360+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |