Overview
- External ontology
- TOGO:M1306 — Modified Reinforced Clostridial Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1306 Original source: JCM - JCM_M1217-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1217
Composition
flowchart LR
CultureMech_007841["modified_reinforced_clostridial_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007841 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007841 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_007841 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_007841 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Glucose["Glucose"]
CultureMech_007841 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Soluble_starch["Soluble starch"]
CultureMech_007841 --> ing5_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing5_Soluble_starch -.-> CHEBI_28017
ing6_agar["agar"]
CultureMech_007841 --> ing6_agar
CHEBI_2509["CHEBI:2509"]
ing6_agar -.-> CHEBI_2509
ing7_Beef_extract["Beef extract"]
CultureMech_007841 --> ing7_Beef_extract
ing8_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
CultureMech_007841 --> ing8_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_H2O -.-> CHEBI_91248
ing9_Tryptone["Tryptone"]
CultureMech_007841 --> ing9_Tryptone
ing10_N2["N2"]
CultureMech_007841 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 3 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
3 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Simple component, Defined component, Organic compound |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 6 | Soluble starch | 1 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 7 | agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Beef extract | 10 G_PER_L | Properties: Undefined component | |
| 9 |
L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 10 | Tryptone | 10 G_PER_L | Properties: Undefined component | |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.327409Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1306 |
| 2026-01-28T19:48:19.640860Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:19:51.886400Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007841 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.325275+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Reinforced Clostridial Medium" to "modified_reinforced_clostridial_medium" for consistent matching |
| 2026-05-16T09:03:09.287593+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.227451+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |