Overview
- External ontology
- TOGO:M1319 — Zestosphaera Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1319 Original source: JCM - JCM_M1227 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1227
Composition
flowchart LR
CultureMech_007855["zestosphaera_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007855 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_007855 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_007855 --> ing2_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing2_Na2S2O3_5H2O -.-> CHEBI_32150
ing3_NaOH["NaOH"]
CultureMech_007855 --> ing3_NaOH
CHEBI_32145["CHEBI:32145"]
ing3_NaOH -.-> CHEBI_32145
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007855 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_Nitrogen_gas["Nitrogen gas"]
CultureMech_007855 --> ing5_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing5_Nitrogen_gas -.-> CHEBI_17997
ing6_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
CultureMech_007855 --> ing6_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_H2O -.-> CHEBI_91248
ing7_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_007855 --> ing7_Casamino_acids__BD_Difco_
ing8_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
CultureMech_007855 --> ing8_Yeast_extract__Oxoid_
ing9_Tryptone["Tryptone"]
CultureMech_007855 --> ing9_Tryptone
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 3 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Simple component, Inorganic compound |
| 4 |
NaOH
HO.Na 39.997 g/mol
|
0.16 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Simple component, Inorganic compound |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 6 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 7 |
L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.0625 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 8 | Casamino acids (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 9 | Yeast extract (Oxoid) | 1 G_PER_L | Properties: Undefined component, Complex component | |
| 10 | Tryptone | 1 G_PER_L | Properties: Undefined component |
Solutions
Modified Brock's salt base solution (see Medium [M156])
Dose: 1 G_PER_L
Cross-reference to Medium M156
Trace mineral solution (see Medium [M447])
Dose: 10 G_PER_L
Cross-reference to Medium M447
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.351247Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1319 |
| 2026-01-28T19:48:15.605817Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:25.775233+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:51.991486Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007855 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.434756+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Zestosphaera Medium" to "zestosphaera_medium" for consistent matching |
| 2026-05-16T09:03:09.374656+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.332775+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |