Overview
- External ontology
- TOGO:M1323 — HLH Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1323 Original source: JCM - JCM_M1231 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1231
Composition
flowchart LR
CultureMech_007860["hlh_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007860 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NH4Cl["NH4Cl"]
CultureMech_007860 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_K2HPO4["K2HPO4"]
CultureMech_007860 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Na2CO3["Na2CO3"]
CultureMech_007860 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_TES["TES"]
CultureMech_007860 --> ing4_TES
CHEBI_39035["CHEBI:39035"]
ing4_TES -.-> CHEBI_39035
ing5_BG_FPC__see_below_["BG-FPC (see below)"]
CultureMech_007860 --> ing5_BG_FPC__see_below_
ing6_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007860 --> ing6_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_7H2O -.-> CHEBI_31795
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007860 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_NaNO3["NaNO3"]
CultureMech_007860 --> ing8_NaNO3
CHEBI_63005["CHEBI:63005"]
ing8_NaNO3 -.-> CHEBI_63005
ing9_EDTA_2Na["EDTA・2Na"]
CultureMech_007860 --> ing9_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing9_EDTA_2Na -.-> CHEBI_64734
ing10_Trace_minerals__see_below_["Trace minerals (see below)"]
CultureMech_007860 --> ing10_Trace_minerals__see_below_
ing11_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007860 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_007860 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007860 --> ing13_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_4H2O -.-> CHEBI_86368
ing14_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_007860 --> ing14_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_7H2O -.-> CHEBI_32312
ing15_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_007860 --> ing15_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_5H2O -.-> CHEBI_31440
ing16_Co_NO3_2_6H2O["Co(NO3)2・6H2O"]
CultureMech_007860 --> ing16_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing16_Co_NO3_2_6H2O -.-> CHEBI_86214
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
792.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 790.0, 1.0, 1.0] |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
30.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
24.4 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 | TES | 2.5 G_PER_L |
CHEBI:39035
TES
|
Role: Buffer |
| 6 | BG-FPC (see below) | 10 G_PER_L | ||
| 7 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
7.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaNO3
NO3.Na 84.994 g/mol
|
149.6 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
0.1 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 11 | Trace minerals (see below) | 100 G_PER_L | ||
| 12 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
390 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
2.9 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
79 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Co(NO3)2・6H2O
Co.6H2O.2NO3 291.031 g/mol
|
49.4 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
8% NaHCO3 solution*
Dose: 10 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
25% Yeast extract solution*
Dose: 10 G_PER_L
Role: Nutrient source; Properties: Undefined component
0.6% Ammonium ferric citrate solution
Dose: 1 G_PER_L
Role: Nitrogen source; Properties: Solution, Defined component, Organic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.359497Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1323 |
| 2026-01-28T19:48:11.099893Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:18.185922+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:45.862816Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:52.030654Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007860 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.474367+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HLH Medium" to "hlh_medium" for consistent matching |
| 2026-05-16T09:03:09.403060+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.362490+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.718316+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.592377+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |