cm_ye_medium_ph_9

CultureMech:007861 bacterial COMPLEX SOLID_AGAR
Original name: CM+YE Medium (pH 9)

Overview

External ontology
TOGO:M1326 — CM+YE Medium (pH 9)
Applications
  • Microbial cultivation
pH
9.0
Notes
TOGO M1326/JCM Medium 1233 prepares CM+YE Medium from TOGO M51/JCM Medium 59, autoclaves it, and adjusts it to pH 9.0 with sterilized 10% Na2CO3 solution.

Composition

flowchart LR
    CultureMech_007861["cm_ye_medium_ph_9"]
    ing0_Casamino_acids__vitamin_assay__BD_Difco_["Casamino acids, vitamin assay (BD-Difco)"]
    CultureMech_007861 --> ing0_Casamino_acids__vitamin_assay__BD_Difco_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007861 --> ing1_Yeast_extract__BD_Difco_
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_007861 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_007861 --> ing3_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing4_KCl["KCl"]
    CultureMech_007861 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_007861 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_007861 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Casamino acids, vitamin assay (BD-Difco) 7.5 G_PER_L TOGO M51 and JCM Medium 59 list this component for CM+YE Medium; TOGO M1326/JCM Medium 1233 adjust the autoclaved medium to pH 9.0.
2 Yeast extract (BD-Difco) 10 G_PER_L TOGO M51 and JCM Medium 59 list this component for CM+YE Medium; TOGO M1326/JCM Medium 1233 adjust the autoclaved medium to pH 9.0.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 TOGO M51 and JCM Medium 59 list this component for CM+YE Medium; TOGO M1326/JCM Medium 1233 adjust the autoclaved medium to pH 9.0.
4 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
 TOGO M51 and JCM Medium 59 list this component for CM+YE Medium; TOGO M1326/JCM Medium 1233 adjust the autoclaved medium to pH 9.0.
5 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
 TOGO M51 and JCM Medium 59 list this component for CM+YE Medium; TOGO M1326/JCM Medium 1233 adjust the autoclaved medium to pH 9.0.
6 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
 TOGO M51 and JCM Medium 59 list this component for CM+YE Medium; TOGO M1326/JCM Medium 1233 adjust the autoclaved medium to pH 9.0.
7 Agar 15 G_PER_L CHEBI:2509
agar
 TOGO M51 and JCM Medium 59 list this component for CM+YE Medium; TOGO M1326/JCM Medium 1233 adjust the autoclaved medium to pH 9.0.

Preparation

  1. Prepare TOGO M51 CM+YE Medium with 1 mL/L Fe(2+) solution.
  2. Autoclave the CM+YE Medium base.
  3. Adjust the autoclaved medium to pH 9.0 with sterilized 10% Na2CO3 solution.

Solutions

Fe(2+) solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 49.8 G_PER_L TOGO M51 and JCM Medium 59 prepare Fe(2+) solution from 4.98 g FeSO4 x 7 H2O in 100 mL distilled water.
TOGO M51 and JCM Medium 59 add 1 mL Fe(2+) solution per liter.

10% Na2CO3 solution

Dose: variable VARIABLE

TOGO and JCM use a sterilized 10% Na2CO3 solution to adjust the autoclaved medium to pH 9.0; the final addition volume is not specified.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.362219Z togo-import Imported from TOGO Medium Source: JCM, ID: M1326
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:05:07.214022+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:52.036058Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007861
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T19:46:10.480600+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CM+YE Medium (pH 9)" to "cm_ye_medium_ph_9" for consistent matching
2026-05-16T09:03:09.409951+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-09-06T00:00:00-07:00 repair_bacterial_togo_score40.py RESOLVED_BACTERIAL_TOGO_SCORE40_GRAPH TOGO M1326/JCM Medium 1233 prepares CM+YE Medium from TOGO M51/JCM Medium 59, autoclaves it, and adjusts it to pH 9.0 with sterilized 10% Na2CO3 solution.