anaerobaculum_medium

CultureMech:007872 bacterial COMPLEX LIQUID
Original name: Anaerobaculum Medium

Overview

External ontology
TOGO:M1336 — Anaerobaculum Medium
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M1336 records JCM Medium 1242 with trypticase peptone, peptone, yeast extract, NaCl, Salt solution from JCM Medium 676, resazurin, distilled water, and an N2 gas atmosphere. JCM 1242 cools the boiled base under N2, adds 5% L-cysteine HCl H2O and adjusts pH to 7.0, autoclaves under N2, then adds autoclaved 1 M glucose and 1 M Na2S2O3 stocks stored under N2.

Composition

flowchart LR
    CultureMech_007872["anaerobaculum_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_007872 --> ing0_Trypticase_peptone
    ing1_Peptone["Peptone"]
    CultureMech_007872 --> ing1_Peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_007872 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_007872 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Resazurin["Resazurin"]
    CultureMech_007872 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Distilled_water["Distilled water"]
    CultureMech_007872 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_N2["N2"]
    CultureMech_007872 --> ing6_N2
    CHEBI_17997["CHEBI:17997"]
    ing6_N2 -.-> CHEBI_17997

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 5.0 G_PER_L JCM Medium 1242 lists 5.0 g/L Trypticase peptone; the product is source-disclosed but not reducible to one ChEBI molecule.
2 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 TOGO M1336 / JCM Medium 1242 lists 5.0 g/L Peptone.
3 Yeast extract 10.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M1336 / JCM Medium 1242 lists 10.0 g/L Yeast extract.
4 NaCl
Cl.Na 58.443 g/mol
8.0 G_PER_L CHEBI:26710
sodium chloride
 TOGO M1336 / JCM Medium 1242 lists 8.0 g/L NaCl.
5 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
 TOGO M1336 / JCM Medium 1242 lists 0.5 mg/L Resazurin.
6 Distilled water
H2O 18.015 g/mol
960.0 ML_PER_L CHEBI:15377
water
 TOGO M1336 / JCM Medium 1242 lists 960.0 ml/L Distilled water.
7 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 JCM Medium 1242 cools the boiled medium under N2, adjusts pH while gassing, and distributes the medium into culture vessels under N2.

Preparation

  1. Mix the base components with Salt solution, bring to a boil, and cool down under an N2 stream.
  2. While gassing, add 5% L-Cysteine HCl H2O solution and adjust to pH 7.0.
  3. Distribute the medium into culture vessels under N2 and seal with butyl rubber stoppers.
  4. Autoclave at 121 degrees C for 15 min.
  5. Cool after autoclaving.
  6. Add autoclaved and N2-stored glucose and Na2S2O3 stock solutions aseptically.

Solutions

Salt solution

Dose: 40.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
CaCl2 x 2H2O 0.25 G_PER_L JCM Medium 676 lists 0.25 g/L CaCl2 x 2H2O.
MgSO4 x 7H2O 0.5 G_PER_L JCM Medium 676 lists 0.5 g/L MgSO4 x 7H2O.
K2HPO4 1.0 G_PER_L JCM Medium 676 lists 1.0 g/L K2HPO4.
KH2PO4 1.0 G_PER_L JCM Medium 676 lists 1.0 g/L KH2PO4.
NaHCO3 10.0 G_PER_L JCM Medium 676 lists 10.0 g/L NaHCO3.
NaCl 2.0 G_PER_L JCM Medium 676 lists 2.0 g/L NaCl.
Distilled water 1.0 L JCM Medium 676 lists 1.0 L Distilled water.
JCM Medium 1242 adds 40.0 ml/L Salt solution and links this stock to the Salt solution printed in JCM Medium 676.

5% L-Cysteine HCl H2O solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl H2O 5.0 PERCENT_W_V 5% L-Cysteine HCl H2O solution is represented from the stock label as 5.0 % w/v L-Cysteine HCl H2O.
JCM Medium 1242 adds 10.0 ml/L 5% L-Cysteine HCl H2O solution while gassing, before pH 7.0 adjustment.

1 M Glucose solution

Dose: 6.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Glucose 1.0 MOLAR 1 M Glucose solution is represented from the stock label as 1.0 M Glucose.
JCM Medium 1242 adds 6.0 ml/L 1 M Glucose solution per liter after autoclaving and cooling.

1 M Na2S2O3 solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2S2O3 1.0 MOLAR 1 M Na2S2O3 solution is represented from the stock label as 1.0 M Na2S2O3.
JCM Medium 1242 adds 10.0 ml/L 1 M Na2S2O3 solution per liter after autoclaving and cooling.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.375444Z togo-import Imported from TOGO Medium Source: JCM, ID: M1336
2026-01-28T19:48:22.518997Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2; Added default concentration for ingredient: N2
2026-03-09T08:04:37.573195+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:09:07.262694Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:52.097168Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007872
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.545838+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Anaerobaculum Medium" to "anaerobaculum_medium" for consistent matching
2026-05-16T09:03:09.457664+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.426523+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1336_score15.py RESOLVED_TOGO_M1336_SCORE15 TOGO M1336 records JCM Medium 1242 with trypticase peptone, peptone, yeast extract, NaCl, Salt solution from JCM Medium 676, resazurin, distilled water, and an N2 gas atmosphere. JCM 1242 cools the boiled base under N2, adds 5% L-cysteine HCl H2O and adjusts pH to 7.0, autoclaves under N2, then adds autoclaved 1 M glucose and 1 M Na2S2O3 stocks stored under N2. Used the JCM Medium 676 salt solution linked from JCM 1242; the imported TOGO wrapper labeled the same stock as Medium M695.