Overview
- External ontology
- TOGO:M1344 — Methylococcaceae IN20 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1344 Original source: JCM - JCM_M1250 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1250
Composition
flowchart LR
CultureMech_007880["methylococcaceae_in20_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007880 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007880 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007880 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007880 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_007880 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_007880 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007880 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_007880 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Na2HPO4["Na2HPO4"]
CultureMech_007880 --> ing8_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing8_Na2HPO4 -.-> CHEBI_34683
ing9_Fe2_SO4_3_nH2O["Fe2(SO4)3・nH2O"]
CultureMech_007880 --> ing9_Fe2_SO4_3_nH2O
ing10_HCl["HCl"]
CultureMech_007880 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_methane["methane"]
CultureMech_007880 --> ing11_methane
CHEBI_16183["CHEBI:16183"]
ing11_methane -.-> CHEBI_16183
ing12_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007880 --> ing12_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_007880 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_007880 --> ing14_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_7H2O -.-> CHEBI_32312
ing15_CoSO4_7H2O["CoSO4・7H2O"]
CultureMech_007880 --> ing15_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_7H2O -.-> CHEBI_91244
ing16_AlK_SO4_2_12H2O["AlK(SO4)2・12H2O"]
CultureMech_007880 --> ing16_AlK_SO4_2_12H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_12H2O -.-> CHEBI_86465
ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_007880 --> ing17_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
ing18_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_007880 --> ing18_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing18_Na2WO4_2H2O -.-> CHEBI_63939
ing19_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_007880 --> ing19_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing19_MnSO4_xH2O -.-> CHEBI_86360
ing20_Na2SeO3["Na2SeO3"]
CultureMech_007880 --> ing20_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing20_Na2SeO3 -.-> CHEBI_48843
ing21_NiCl2_5H2O["NiCl2・5H2O"]
CultureMech_007880 --> ing21_NiCl2_5H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_5H2O -.-> CHEBI_34887
ing22_KOH["KOH"]
CultureMech_007880 --> ing22_KOH
CHEBI_32035["CHEBI:32035"]
ing22_KOH -.-> CHEBI_32035
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.25 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 | Fe2(SO4)3・nH2O | 0.02 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 11 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
| 12 |
methane
CH4 16.043 g/mol
|
variable VARIABLE |
CHEBI:16183
methane
|
Properties: Gas |
| 13 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.36 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoSO4・7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
AlK(SO4)2・12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 19 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.025 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
1 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.3 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
NiCl2・5H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 23 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
1 mM CuSO4・5H2O solution
Dose: 0.1 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
Trace mineral solution (see below)
Dose: 3.3 G_PER_L
8% NaHCO3 solution
Dose: 1.25 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.391729Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1344 |
| 2026-01-28T19:48:27.927793Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl; Added default concentration for ingredient: methane; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:46.206966+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:16.751996Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:52.169840Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007880 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.617786+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methylococcaceae IN20 Medium" to "methylococcaceae_in20_medium" for consistent matching |
| 2026-05-16T09:03:09.532052+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.505151+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.838186+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.634579+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:18.231539+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-02T07:37:44.065943+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |