Overview
- External ontology
- TOGO:M1361 — GBS Salts Medium For Thermanaeromonas Inyoensis
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1361 Original source: JCM - JCM_M1265 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1265
Composition
flowchart LR
CultureMech_007899["gbs_salts_medium_for_thermanaeromonas_inyoensis"]
ing0_Distilled_water["Distilled water"]
CultureMech_007899 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007899 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007899 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007899 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_007899 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KCl["KCl"]
CultureMech_007899 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2SO4["Na2SO4"]
CultureMech_007899 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_NaH2PO4["NaH2PO4"]
CultureMech_007899 --> ing7_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing7_NaH2PO4 -.-> CHEBI_37585
ing8_N2["N2"]
CultureMech_007899 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.123 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.015 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.107 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.15 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.3 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
1.38 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Mineral solution (see Medium [M1029])
Dose: 5 G_PER_L
Cross-reference to Medium M1029
8.0% NaHCO3 solution*
Dose: 2 G_PER_L
Role: Buffer; Properties: Solution, Inorganic compound, Defined component
1.0 M Sodium lactate solution
Dose: 5 G_PER_L
Role: Carbon source; Properties: Solution, Organic compound, Defined component
1.0 M Sodium acetate solution
Dose: 2 G_PER_L
Role: Buffer; Properties: Defined component, Solution, Organic compound
5% Na2S・9H2O solution
Dose: 2 G_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
1.0 M Sodium thiosulfate solution
Dose: 2 G_PER_L
Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.429382Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1361 |
| 2026-01-28T19:48:26.580157Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:44.134231+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T06:19:52.331555Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007899 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.787170+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GBS Salts Medium For Thermanaeromonas Inyoensis" to "gbs_salts_medium_for_thermanaeromonas_inyoensis" for consistent matching |
| 2026-05-16T09:03:09.682935+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.680039+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.973754+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.688585+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |