Overview
- External ontology
- TOGO:M1365 — Artificial Seawater Yeast Extract-Tryptone Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1365 Original source: JCM - JCM_M1269 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1269
Composition
flowchart LR
CultureMech_007903["artificial_seawater_yeast_extract_tryptone_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007903 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007903 --> ing1_Yeast_extract
ing2_Tryptone["Tryptone"]
CultureMech_007903 --> ing2_Tryptone
ing3_Artificial_seawater__4x__see_below_["Artificial seawater (4x, see below)"]
CultureMech_007903 --> ing3_Artificial_seawater__4x__see_below_
ing4_N2["N2"]
CultureMech_007903 --> ing4_N2
CHEBI_17997["CHEBI:17997"]
ing4_N2 -.-> CHEBI_17997
ing5_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007903 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_007903 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007903 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_K2HPO4["K2HPO4"]
CultureMech_007903 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007903 --> ing9_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_6H2O -.-> CHEBI_86345
ing10_KCl["KCl"]
CultureMech_007903 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_NaHCO3["NaHCO3"]
CultureMech_007903 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12__NH4_2SO4["(NH4)2SO4"]
CultureMech_007903 --> ing12__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing12__NH4_2SO4 -.-> CHEBI_62946
ing13_NaBr["NaBr"]
CultureMech_007903 --> ing13_NaBr
CHEBI_63004["CHEBI:63004"]
ing13_NaBr -.-> CHEBI_63004
ing14_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_007903 --> ing14_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing14_SrCl2_6H2O -.-> CHEBI_36385
ing15_Ammonium_Fe_III__citrate["Ammonium Fe(III) citrate"]
CultureMech_007903 --> ing15_Ammonium_Fe_III__citrate
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
801.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 800.0, 1.0] |
| 2 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 | Tryptone | 2 G_PER_L | Properties: Undefined component | |
| 4 | Artificial seawater (4x, see below) | 200 G_PER_L | ||
| 5 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 6 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
24 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
80 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.68 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
12 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.08 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 | Ammonium Fe(III) citrate | 0.04 G_PER_L | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.437486Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1365 |
| 2026-01-28T19:48:41.989315Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T03:09:42.579301Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:52.367883Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007903 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.824492+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Artificial Seawater Yeast Extract-Tryptone Medium" to "artificial_seawater_yeast_extract_tryptone_medium" for consistent matching |
| 2026-05-16T09:03:24.974311+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:49.815447+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:36.832902+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:53.565742+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |