chocolate_agar_with_5_sheep_blood

CultureMech:007904 bacterial COMPLEX SOLID_AGAR
Original name: Chocolate Agar With 5% Sheep Blood

Overview

External ontology
TOGO:M1366 — Chocolate Agar With 5% Sheep Blood
Applications
  • Microbial cultivation
Notes
TOGO M1366 mirrors JCM Medium 1270: 44.0 g Columbia blood agar base (BD-Difco), 50.0 ml sheep blood, and 950.0 ml distilled water.

Composition

flowchart LR
    CultureMech_007904["chocolate_agar_with_5_sheep_blood"]
    ing0_Columbia_blood_agar_base__BD_Difco_["Columbia blood agar base (BD-Difco)"]
    CultureMech_007904 --> ing0_Columbia_blood_agar_base__BD_Difco_
    ing1_Sheep_blood["Sheep blood"]
    CultureMech_007904 --> ing1_Sheep_blood
    ing2_Distilled_water["Distilled water"]
    CultureMech_007904 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Columbia blood agar base (BD-Difco) 44.0 G_PER_L JCM Medium 1270 lists 44.0 g Columbia blood agar base (BD-Difco).
2 Sheep blood 50.0 ML_PER_L UBERON:0000178
blood
 JCM Medium 1270 lists 50.0 ml sheep blood.
3 Distilled water
H2O 18.015 g/mol
950.0 ML_PER_L CHEBI:15377
water
 JCM Medium 1270 lists 950.0 ml distilled water.

Preparation

  1. Mix 44.0 g Columbia blood agar base (BD-Difco) with 950.0 ml distilled water.
  2. Autoclave and cool to about 70 C.
  3. Aseptically add 50.0 ml sterile defibrinated sheep blood and keep at 70 C for 15 min.
  4. Cool to about 50 C.
  5. Mix and quickly dispense into sterile petri dishes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.439562Z togo-import Imported from TOGO Medium Source: JCM, ID: M1366
2026-01-28T19:48:34.059512Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:52.372995Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007904
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.829897+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Chocolate Agar With 5% Sheep Blood" to "chocolate_agar_with_5_sheep_blood" for consistent matching
2026-06-05T07:40:32.719325+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_official_simple_score20.py RESOLVED_OFFICIAL_SIMPLE_SCORE20 TOGO M1366 mirrors JCM Medium 1270: 44.0 g Columbia blood agar base (BD-Difco), 50.0 ml sheep blood, and 950.0 ml distilled water.