Overview
- External ontology
- TOGO:M1371 — Sulfolobus Medium With Tryptone
- Applications
-
- Microbial cultivation
- pH
- 1.5
- Notes
- JCM Medium 1275 adds 0.5 g/L Tryptone to the JCM 165 Modified Brock's salt base solution and adjusts pH to 1.5 with H2SO4.
Composition
flowchart LR
CultureMech_007910["sulfolobus_medium_with_tryptone"]
ing0_Tryptone["Tryptone"]
CultureMech_007910 --> ing0_Tryptone
ing1_H2SO4["H2SO4"]
CultureMech_007910 --> ing1_H2SO4
CHEBI_26836["CHEBI:26836"]
ing1_H2SO4 -.-> CHEBI_26836
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 0.5 G_PER_L | JCM Medium 1275 lists 0.5 g/L Tryptone. | |
| 2 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
JCM Medium 1275 adjusts pH to 1.5 with H2SO4. |
Preparation
- Prepare Modified Brock's salt base solution from JCM Medium 165 and add Tryptone.
- Adjust pH to 1.5 with H2SO4.
Solutions
Modified Brock's salt base solution
Dose: 1000.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| (NH4)2SO4 | 1.3 G_PER_L | JCM Medium 165 prints this component in its stock solution. |
| KH2PO4 | 0.28 G_PER_L | JCM Medium 165 prints this component in its stock solution. |
| MgSO4 x 7H2O | 0.25 G_PER_L | JCM Medium 165 prints this component in its stock solution. |
| CaCl2 x 2H2O | 0.07 G_PER_L | JCM Medium 165 prints this component in its stock solution. |
| FeCl3 x 6H2O | 2.0 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| MnCl2 x 4H2O | 1.8 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| Na2B4O7 x 10H2O | 4.5 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| ZnSO4 x 7H2O | 0.22 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| CuCl2 x 2H2O | 0.05 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| Na2MoO4 x 2H2O | 0.03 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| VOSO4 x H2O | 0.03 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| CoSO4 x 7H2O | 0.01 MG_PER_L | JCM Medium 165 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 165 prints this component in its stock solution. |
JCM Medium 1275 uses 1.0 L Modified Brock's salt base solution from JCM Medium 165.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.447354Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1371 |
| 2026-01-28T19:48:10.965385Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:04:17.938828+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:52.413310Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007910 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.871933+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sulfolobus Medium With Tryptone" to "sulfolobus_medium_with_tryptone" for consistent matching |
| 2026-05-16T09:03:09.765881+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.769101+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_togo_jcm_archaea_score15.py | RESOLVED_TOGO_JCM_ARCHAEA_SCORE15 | JCM Medium 1275 adds 0.5 g/L Tryptone to the JCM 165 Modified Brock's salt base solution and adjusts pH to 1.5 with H2SO4. |