Overview
- External ontology
- TOGO:M138 — Alkaline Malt Extract Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M138 Original source: JCM - JCM_M147 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=147
Composition
flowchart LR
CultureMech_007924["alkaline_malt_extract_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_007924 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Agar["Agar"]
CultureMech_007924 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Malt_extract["Malt extract"]
CultureMech_007924 --> ing2_Malt_extract
ing3_Peptone["Peptone"]
CultureMech_007924 --> ing3_Peptone
ing4_NaOH["NaOH"]
CultureMech_007924 --> ing4_NaOH
CHEBI_32145["CHEBI:32145"]
ing4_NaOH -.-> CHEBI_32145
ing5_Na2CO3["Na2CO3"]
CultureMech_007924 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 100.0] |
| 2 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 3 | Malt extract | 10 G_PER_L | Properties: Undefined component | |
| 4 | Peptone | 2.5 G_PER_L | ||
| 5 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
6 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
Solutions
Solution A
Dose: 900 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
NaHCO3 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.461501Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M138 |
| 2026-01-28T19:48:29.626758Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: NaHCO3 solution |
| 2026-03-09T08:04:49.221125+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:20.356357Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:52.522630Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007924 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.985059+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Malt Extract Agar" to "alkaline_malt_extract_agar" for consistent matching |
| 2026-05-16T09:03:23.654230+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:47.892964+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.804991+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |