Overview
- External ontology
- TOGO:M1390 — MMJ/KT2 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1390 Original source: JCM - JCM_M1294 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1294 Post-inoculation instruction: Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Composition
flowchart LR
CultureMech_007925["mmj_kt2_medium"]
ing0_Resazurin["Resazurin"]
CultureMech_007925 --> ing0_Resazurin
CHEBI_8806["CHEBI:8806"]
ing0_Resazurin -.-> CHEBI_8806
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007925 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_N2["N2"]
CultureMech_007925 --> ing2_N2
CHEBI_17997["CHEBI:17997"]
ing2_N2 -.-> CHEBI_17997
ing3_Hydrogen_gas["Hydrogen gas"]
CultureMech_007925 --> ing3_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing3_Hydrogen_gas -.-> CHEBI_18276
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound Source batch input: 1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 3 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 4 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Preparation
- Adjust pH to 7.0 and autoclave. After cooling, add the following solutions (filter-sterilized):
- Aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under N2):
Solutions
Modified MJ synthetic seawater C
Dose: 1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1 L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component Source water batch input: 1 L = 1 L. This is not a measured final volume. Prior duplicate-collapse notes describe the superseded flattened record. |
| MgSO4・7H2O | Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component Source batch input: 3.4 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| NaCl | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component Source batch input: 25 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| CaCl2・2H2O | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound Source batch input: 0.7 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| NH4Cl | Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound Source batch input: 0.25 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| K2HPO4 | Role: Buffer; Properties: Simple component, Inorganic compound, Defined component Source batch input: 0.14 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| MgCl2・6H2O | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound Source batch input: 4.2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| FeSO4・7H2O | Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component Source batch input: 0.02 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| KCl | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. |
Source stock addition: 1 L = 1 L per receiving batch, not grams per liter or a dose per final liter.
Trace mineral solution (see below)
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| NiCl2・6H2O | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound Source batch input: 0.075 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| Na2SeO3・5H2O | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component Source batch input: 0.05 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| Na2WO4・2H2O | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound Source batch input: 0.1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. |
Source stock addition: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter.
Trace minerals (see Medium [M142])
Dose: 1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1 L | Source water batch input: 1 L = 1 L. This is not a measured final volume. Prior duplicate-collapse notes describe the superseded flattened record. |
| MgSO4・7H2O | Source batch input: 3 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| NaCl | Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| CaCl2・2H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| Na2MoO4・2H2O | Source batch input: 0.01 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| H3BO3 | Source batch input: 0.01 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| FeSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| ZnSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| CuSO4・5H2O | Source batch input: 0.01 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| CoSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| Nitrilotriacetic acid | Source batch input: 1.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| MnSO4・xH2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| AlK(SO4)2 | Source batch input: 0.01 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. |
Cross-reference to Medium M142
Source stock addition: 1 L = 1 L per receiving batch, not grams per liter or a dose per final liter.
KOH solution
Stock pH-adjustment reagent with no fixed dose or numeric strength.
8% NaHCO3 solution
Dose: 0.0125 L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Source stock addition: 12.5 ml = 0.0125 L per receiving batch, not grams per liter or a dose per final liter. Percentage basis is unspecified; no numeric stock strength is asserted.
Trace vitamins (see Medium [M190])
Dose: 0.01 L
Cross-reference to Medium M190
Source stock addition: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter.
Vitamin B12 solution (see Medium [M401])
Dose: 0.0002 L
Cross-reference to Medium M401
Source stock addition: 0.2 ml = 0.0002 L per receiving batch, not grams per liter or a dose per final liter.
5% Na2S・9H2O solution
Dose: 10 ML_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
Source stock addition: 10 ml; explicitly 10 ml per receiving-medium liter. Percentage basis is unspecified; no numeric stock strength is asserted.
5% L-Cysteine・HCl・H2O solution
Dose: 10 ML_PER_L
Properties: Defined component, Organic compound, Solution
Source stock addition: 10 ml; explicitly 10 ml per receiving-medium liter. Percentage basis is unspecified; no numeric stock strength is asserted.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.473925Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1390 |
| 2026-01-28T19:48:16.013566Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:26.423817+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:08:55.014987Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:52.532308Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007925 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.994660+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ/KT2 Medium" to "mmj_kt2_medium" for consistent matching |
| 2026-05-16T09:03:09.893559+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.898495+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:23.154155+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.763224+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T15:19:50.373672+00:00 | codex | RESTORED_NESTED_M142_TRACE_STOCKS_WITH_EVIDENCE | Restored source-specific nested stocks, batch/per-liter addition bases and explicit stock strengths. Preserved original annotations and history; unsupported final-medium mass concentrations are withdrawn with source batch amounts retained in notes. Gas placeholders and unverified chemical groundings remain unresolved. |
| 2026-10-08T16:02:54.837783+00:00 | codex | EXPANDED_LITERAL_RECIPE_EVIDENCE_EXCERPT | Expanded an excerpt that ended within the word 'solutions'. No ingredient, concentration, unit, scope, or calculation changed. Source locator: preparation paragraph; accessed=2026-10-08T14:49:33.670783+00:00; source SHA256=a68b7668a752a8c7e30a6401b902cded045d9ff2644573b1e703e342e4581792. |