alkaline_clostridial_nutrient_medium

CultureMech:007927 bacterial COMPLEX LIQUID
Original name: Alkaline Clostridial Nutrient Medium

Overview

External ontology
TOGO:M1392 — Alkaline Clostridial Nutrient Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1392 Original source: JCM - JCM_M1295-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1295

Composition

flowchart LR
    CultureMech_007927["alkaline_clostridial_nutrient_medium"]
    ing0_N2["N2"]
    CultureMech_007927 --> ing0_N2
    CHEBI_17997["CHEBI:17997"]
    ing0_N2 -.-> CHEBI_17997
    ing1_Distilled_water["Distilled water"]
    CultureMech_007927 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_007927 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_007927 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Sodium_acetate["Sodium acetate"]
    CultureMech_007927 --> ing4_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate -.-> CHEBI_32954
    ing5_gellan_gum["gellan gum"]
    CultureMech_007927 --> ing5_gellan_gum
    CHEBI_85248["CHEBI:85248"]
    ing5_gellan_gum -.-> CHEBI_85248
    ing6_Glucose["Glucose"]
    CultureMech_007927 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Soluble_starch["Soluble starch"]
    CultureMech_007927 --> ing7_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing7_Soluble_starch -.-> CHEBI_28017
    ing8_Beef_extract["Beef extract"]
    CultureMech_007927 --> ing8_Beef_extract
    ing9_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
    CultureMech_007927 --> ing9_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing10_Peptone["Peptone"]
    CultureMech_007927 --> ing10_Peptone
    ing11_NaHCO3["NaHCO3"]
    CultureMech_007927 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Na2CO3["Na2CO3"]
    CultureMech_007927 --> ing12_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing12_Na2CO3 -.-> CHEBI_29377

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
2 Distilled water
H2O 18.015 g/mol
1000.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 900.0, 100.0]
3 Yeast extract 3 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 Sodium acetate
C2H3O2.Na 82.034 g/mol
3 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
6 gellan gum 10 G_PER_L CHEBI:85248
gellan gum
 Role: Solidifying component; Properties: Undefined component, Organic compound
7 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
8 Soluble starch 1 G_PER_L CHEBI:28017
starch
 Role: Carbon source; Properties: Undefined component
9 Beef extract 10 G_PER_L Properties: Undefined component
10 L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
11 Peptone 5 G_PER_L
12 NaHCO3
CHO3.Na 84.006 g/mol
2.9 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
13 Na2CO3
CO3.2Na 105.988 g/mol
7 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component

Solutions

Solution A

Dose: 900 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 100 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.478426Z togo-import Imported from TOGO Medium Source: JCM, ID: M1392
2026-01-28T19:48:10.947199Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:17.907262+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:08:45.553250Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:52.550327Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007927
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.012836+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Alkaline Clostridial Nutrient Medium" to "alkaline_clostridial_nutrient_medium" for consistent matching
2026-05-16T09:03:09.914051+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.919494+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.773168+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.