abss2

CultureMech:007930 bacterial COMPLEX LIQUID
Original name: ABSS2

Overview

External ontology
TOGO:M1395 — ABSS2
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1395 Original source: JCM - JCM_M1298 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1298

Composition

flowchart LR
    CultureMech_007930["abss2"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007930 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007930 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007930 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007930 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_007930 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007930 --> ing5_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing5_Carbon_dioxide_gas -.-> CHEBI_16526
    ing6_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007930 --> ing6_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing6_Nitrogen_gas -.-> CHEBI_17997
    ing7_Biotin["Biotin"]
    CultureMech_007930 --> ing7_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing7_Biotin -.-> CHEBI_15956
    ing8_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_007930 --> ing8_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing8_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing9_Thiamine_HCl["Thiamine-HCl"]
    CultureMech_007930 --> ing9_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing9_Thiamine_HCl -.-> CHEBI_49105
    ing10_Folic_acid["Folic acid"]
    CultureMech_007930 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Vitamin_B12["Vitamin B12"]
    CultureMech_007930 --> ing11_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing11_Vitamin_B12 -.-> CHEBI_176843
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_007930 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_Inositol["Inositol"]
    CultureMech_007930 --> ing13_Inositol
    CHEBI_24848["CHEBI:24848"]
    ing13_Inositol -.-> CHEBI_24848
    ing14_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_007930 --> ing14_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing14_DL_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0]
2 NaCl
Cl.Na 58.443 g/mol
11.8 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.3 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
2.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 KCl
Cl.K 74.551 g/mol
0.4 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
7 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
8 Biotin
C10H16N2O3S 244.316 g/mol
10 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
9 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
100 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
10 Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
2 G_PER_L CHEBI:49105
thiamine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
11 Folic acid
C19H19N7O6 441.404 g/mol
10 G_PER_L CHEBI:27470
folic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 Vitamin B12 10 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13 Nicotinic acid
C6H5NO2 123.111 g/mol
1 G_PER_L CHEBI:15940
nicotinic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Inositol
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:24848
inositol
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
15 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
1 G_PER_L CHEBI:31345
Calcium pantothenate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

1 M NaHCO3 solution*

Dose: 3 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

100 mM NH4Cl solution

Dose: 20 G_PER_L

Role: Nitrogen source; Properties: Defined component, Inorganic compound, Solution

10 mM Na2HPO4 solution

Dose: 100 G_PER_L

Role: Buffer; Properties: Solution, Inorganic compound, Defined component

500 mM MnO2 solution

Dose: 10 G_PER_L

Role: Mineral source

300 mM Na2S2O3 solution

Dose: 10 G_PER_L

Properties: Defined component, Inorganic compound, Solution

SL--7 trace element solution (see Medium [M538])

Dose: 100 G_PER_L

Cross-reference to Medium M538

Vitamin solution* (see below)

Dose: 420 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.483372Z togo-import Imported from TOGO Medium Source: JCM, ID: M1395
2026-01-28T19:48:39.986287Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:05:07.325851+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 7 solution(s) from ingredients
2026-03-13T03:09:39.041992Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:52.573187Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007930
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.036635+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ABSS2" to "abss2" for consistent matching
2026-05-16T09:03:09.938106+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.946604+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-02T07:37:44.339089+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.