Overview
- External ontology
- TOGO:M1399 — Sea Salts YTG Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M1399 imports JCM Medium 1302. JCM 1302 records the pH 7.0 Sea Salts YTG base, 1.0 ml/L Trace minerals from JCM Medium 151, and filter-sterilized post-autoclave additions of 10.0 ml/L Trace vitamins from JCM Medium 197 and 5.0 ml/L 1 M Sodium thiosulfate.
Composition
flowchart LR
CultureMech_007934["sea_salts_ytg_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_007934 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_007934 --> ing1_Tryptone
ing2_Glucose["Glucose"]
CultureMech_007934 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sea_salts__Sigma_Aldrich_["Sea salts (Sigma-Aldrich)"]
CultureMech_007934 --> ing3_Sea_salts__Sigma_Aldrich_
ing4_L_Cysteine_HCl_H2O["L-Cysteine HCl H2O"]
CultureMech_007934 --> ing4_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_H2O -.-> CHEBI_91248
ing5_Resazurin["Resazurin"]
CultureMech_007934 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Distilled_water["Distilled water"]
CultureMech_007934 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_N2["N2"]
CultureMech_007934 --> ing7_N2
CHEBI_17997["CHEBI:17997"]
ing7_N2 -.-> CHEBI_17997
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M1399 / JCM Medium 1302 lists 0.5 g/L Yeast extract. |
| 2 | Tryptone | 1.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
TOGO M1399 / JCM Medium 1302 lists 1.0 g/L Tryptone. |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
TOGO M1399 / JCM Medium 1302 lists 2.5 g/L Glucose. |
| 4 | Sea salts (Sigma-Aldrich) | 30.0 G_PER_L | JCM Medium 1302 lists 30.0 g/L Sea salts (Sigma-Aldrich); this commercial mixture is source-disclosed but not reducible to one ChEBI molecule. | |
| 5 |
L-Cysteine HCl H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.2 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
TOGO M1399 / JCM Medium 1302 lists 0.2 g/L L-Cysteine HCl H2O. |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
TOGO M1399 / JCM Medium 1302 lists 0.5 mg/L Resazurin. |
| 7 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 1302 brings the base medium to 1.0 L with distilled water. |
| 8 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1302 dispenses the base into culture vessels under a N2 gas atmosphere and adds filter-sterilized stocks anaerobically after cooling. |
Preparation
- Mix the base components with Trace minerals and 0.1% Na2SeO3 solution and bring the volume to 1.0 L.
- Adjust the base medium to pH 7.0.
- Dispense the medium into culture vessels under N2 gas, for example 10 ml medium in Hungate tubes, and seal with butyl rubber stoppers.
- Autoclave at 121 degrees C for 15 min.
- Cool after autoclaving.
- Aseptically and anaerobically add filter-sterilized Trace vitamins and 1 M Sodium thiosulfate stocks per liter.
Solutions
Trace minerals
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.5 G_PER_L | JCM Medium 151 lists 1.5 g/L Nitrilotriacetic acid. |
| MgSO4 x 7 H2O | 3.0 G_PER_L | JCM Medium 151 lists 3.0 g/L MgSO4 x 7 H2O. |
| MnSO4 x n H2O | 0.5 G_PER_L | JCM Medium 151 lists 0.5 g/L MnSO4 x n H2O. |
| NaCl | 1.0 G_PER_L | JCM Medium 151 lists 1.0 g/L NaCl. |
| FeSO4 x 7 H2O | 0.1 G_PER_L | JCM Medium 151 lists 0.1 g/L FeSO4 x 7 H2O. |
| CoSO4 x 7 H2O | 0.1 G_PER_L | JCM Medium 151 lists 0.1 g/L CoSO4 x 7 H2O. |
| CaCl2 x 2 H2O | 0.1 G_PER_L | JCM Medium 151 lists 0.1 g/L CaCl2 x 2 H2O. |
| ZnSO4 x 7 H2O | 0.1 G_PER_L | JCM Medium 151 lists 0.1 g/L ZnSO4 x 7 H2O. |
| CuSO4 x 5 H2O | 0.01 G_PER_L | JCM Medium 151 lists 0.01 g/L CuSO4 x 5 H2O. |
| AlK(SO4)2 | 0.01 G_PER_L | JCM Medium 151 lists 0.01 g/L AlK(SO4)2. |
| H3BO3 | 0.01 G_PER_L | JCM Medium 151 lists 0.01 g/L H3BO3. |
| Na2MoO4 x 2 H2O | 0.01 G_PER_L | JCM Medium 151 lists 0.01 g/L Na2MoO4 x 2 H2O. |
| Distilled water | 1.0 L | JCM Medium 151 lists 1.0 L Distilled water. |
| KOH | variable VARIABLE | JCM Medium 151 uses KOH solution to adjust the Trace minerals stock to pH 6.5 before mineral addition and to final pH 7.0. |
JCM Medium 1302 adds 1.0 ml/L Trace minerals and links this stock to the Trace minerals recipe printed in JCM Medium 151.
0.1% Na2SeO3 solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2SeO3 | 0.1 PERCENT_W_V | TOGO M1399 / JCM Medium 1302 lists 0.1 % w/v Na2SeO3. |
JCM Medium 1302 adds 1.0 ml/L 0.1% Na2SeO3 solution.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine hydrochloride | 10.0 MG_PER_L | JCM Medium 197 lists 10.0 mg/L Pyridoxine hydrochloride. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1.0 L | JCM Medium 197 lists 1.0 L Distilled water. |
JCM Medium 1302 adds 10.0 ml/L filter-sterilized Trace vitamins from the stock recipe printed in JCM Medium 197 after autoclaving.
1 M Sodium thiosulfate solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium thiosulfate | 1.0 MOLAR | TOGO M1399 / JCM Medium 1302 lists 1.0 M Sodium thiosulfate. |
JCM Medium 1302 adds 5.0 ml/L filter-sterilized 1 M Sodium thiosulfate solution after autoclaving.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.491535Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1399 |
| 2026-01-28T19:48:38.124433Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:03.749840+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:19:52.605380Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007934 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.069812+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sea Salts YTG Medium" to "sea_salts_ytg_medium" for consistent matching |
| 2026-05-16T09:03:09.976931+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.985762+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1399_score15.py | RESOLVED_TOGO_M1399_SCORE15 | TOGO M1399 imports JCM Medium 1302. JCM 1302 records the pH 7.0 Sea Salts YTG base, 1.0 ml/L Trace minerals from JCM Medium 151, and filter-sterilized post-autoclave additions of 10.0 ml/L Trace vitamins from JCM Medium 197 and 5.0 ml/L 1 M Sodium thiosulfate. MediaDive J1302 confirms the same post-autoclave 10.0 ml/L Trace vitamins and 5.0 ml/L Sodium thiosulfate additions; corrected the imported stock additions from g/L artifacts to ml/L volumes. |