Overview
- External ontology
- TOGO:M1422 — Rust fungi Medium
- Applications
-
- Microbial cultivation
- pH
- 5.8
- Notes
- TOGO M1422 imports NBRC Medium 19 as Rust fungi Medium. NBRC Medium 19 lists 1.15 g Murashige & Skoog's plant salt mixture from Wako Pure Chemicals, 2 g Peptic Pepton from USB, 2 g Lab-Lemco Broth from Oxoid, 40 g sucrose, distilled water to 1 L, and 20 g Bacto Agar from Difco at pH 5.8.
Composition
flowchart LR
CultureMech_007960["rust_fungi_medium"]
ing0_Murashige_and_Skoog_plant_salt_mixture__Wako_["Murashige and Skoog plant salt mixture (Wako)"]
CultureMech_007960 --> ing0_Murashige_and_Skoog_plant_salt_mixture__Wako_
ing1_Peptic_Pepton__USB_["Peptic Pepton (USB)"]
CultureMech_007960 --> ing1_Peptic_Pepton__USB_
ing2_Lab_Lemco_Broth__Oxoid_["Lab-Lemco Broth (Oxoid)"]
CultureMech_007960 --> ing2_Lab_Lemco_Broth__Oxoid_
ing3_Sucrose["Sucrose"]
CultureMech_007960 --> ing3_Sucrose
CHEBI_17992["CHEBI:17992"]
ing3_Sucrose -.-> CHEBI_17992
ing4_Distilled_water["Distilled water"]
CultureMech_007960 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_Bacto_Agar__Difco_["Bacto Agar (Difco)"]
CultureMech_007960 --> ing5_Bacto_Agar__Difco_
CHEBI_2509["CHEBI:2509"]
ing5_Bacto_Agar__Difco_ -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Murashige and Skoog plant salt mixture (Wako) | 1.15 G_PER_L | NBRC Medium 19 lists 1.15 g/L Murashige & Skoog's plant salt mixture, with a source footnote naming Wako Pure Chemicals; this commercial blend is retained as an opaque complex component. | |
| 2 | Peptic Pepton (USB) | 2.0 G_PER_L | NBRC Medium 19 lists 2.0 g/L Peptic Pepton from USB; this brand-specific peptone is retained as an opaque complex component. | |
| 3 | Lab-Lemco Broth (Oxoid) | 2.0 G_PER_L | NBRC Medium 19 lists 2.0 g/L Lab-Lemco Broth from Oxoid; this brand-specific broth powder is retained as an opaque complex component. | |
| 4 |
Sucrose
C12H22O11 342.297 g/mol
|
40.0 G_PER_L |
CHEBI:17992
sucrose
|
NBRC Medium 19 lists 40.0 g/L Sucrose. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
NBRC Medium 19 lists Distilled water to 1 L. |
| 6 | Bacto Agar (Difco) | 20.0 G_PER_L |
CHEBI:2509
agar
|
NBRC Medium 19 lists 20.0 g/L Bacto Agar from Difco. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.661957Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1422 |
| 2026-01-28T19:48:09.085014Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:14.607425+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:52.731104Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007960 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.204306+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Rust fungi Medium" to "rust_fungi_medium" for consistent matching |
| 2026-05-16T09:04:31.032087+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:44.900741+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m1422_rust_fungi_score15.py | RESOLVED_TOGO_M1422_RUST_FUNGI_SCORE15 | TOGO M1422 imports NBRC Medium 19 as Rust fungi Medium. NBRC Medium 19 lists 1.15 g Murashige & Skoog's plant salt mixture from Wako Pure Chemicals, 2 g Peptic Pepton from USB, 2 g Lab-Lemco Broth from Oxoid, 40 g sucrose, distilled water to 1 L, and 20 g Bacto Agar from Difco at pH 5.8. Corrected the imported water unit, set pH 5.8, moved Murashige and Skoog plant salt mixture back from an empty solution to the ingredient list, grounded exact water, sucrose, and agar components, and retained the brand-specific complex powders as unmapped ingredients. |