Overview
- External ontology
- TOGO:M1444 — TOGO Medium M1444
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1444 imports NBRC Medium 209: SI Medium Dehydrated from Nippon Jozo Kyokai, ethyl alcohol, and 900 ml distilled water. NBRC footnotes the ethanol addition: sterilize it by filtration and add it to autoclaved SI medium.
Composition
flowchart LR
CultureMech_007984["togo_medium_m1444"]
ing0_SI_Medium_Dehydrated["SI Medium Dehydrated"]
CultureMech_007984 --> ing0_SI_Medium_Dehydrated
ing1_Ethyl_alcohol["Ethyl alcohol"]
CultureMech_007984 --> ing1_Ethyl_alcohol
CHEBI_16236["CHEBI:16236"]
ing1_Ethyl_alcohol -.-> CHEBI_16236
ing2_Distilled_water["Distilled water"]
CultureMech_007984 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | SI Medium Dehydrated | 50 G_PER_L | NBRC Medium 209 lists 50 g/L SI Medium Dehydrated from Nippon Jozo Kyokai, Tokyo; this dehydrated commercial medium is retained as an opaque complex component. | |
| 2 |
Ethyl alcohol
C2H6O 46.069 g/mol
|
100 ML_PER_L |
CHEBI:16236
ethanol
|
NBRC Medium 209 lists 100 ml/L ethyl alcohol; the source footnote says to sterilize it by filtration and add it to SI medium sterilized by autoclaving. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
900 ML_PER_L |
CHEBI:15377
water
|
NBRC Medium 209 lists 900 ml/L distilled water. |
Preparation
- Autoclave 50 g/L SI Medium Dehydrated in 900 ml/L distilled water.
- Sterilize 100 ml/L ethyl alcohol by filtration.
- Add the filter-sterilized ethyl alcohol to the autoclaved SI medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.684571Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1444 |
| 2026-01-28T19:48:16.873466Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:27.885024+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:52.839696Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007984 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.321786+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "TOGO Medium M1444" to "togo_medium_m1444" for consistent matching |
| 2026-05-16T09:04:35.970557+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:52.199295+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_nbrc_209_score15.py | RESOLVED_NBRC_209_SCORE15 | Curated TOGO:M1444 from TOGO and NBRC Medium 209; moved the SI Medium Dehydrated and ethyl alcohol rows out of empty solution wrappers, corrected volume rows to ml/L, grounded ethanol and water, and added autoclave and filter-sterilization steps. |