hydrogenobacter_thermophilus_medium

CultureMech:008001 bacterial COMPLEX LIQUID
Original name: Hydrogenobacter Thermophilus Medium

Overview

External ontology
TOGO:M145 — Hydrogenobacter Thermophilus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M145 Original source: JCM - JCM_M154 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=154

Composition

flowchart LR
    CultureMech_008001["hydrogenobacter_thermophilus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008001 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_008001 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_008001 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008001 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_008001 --> ing4_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_7H2O -.-> CHEBI_75836
    ing5_CaCl2["CaCl2"]
    CultureMech_008001 --> ing5_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing5_CaCl2 -.-> CHEBI_3312
    ing6_Na2HPO4["Na2HPO4"]
    CultureMech_008001 --> ing6_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing6_Na2HPO4 -.-> CHEBI_34683
    ing7_NH4NO3["NH4NO3"]
    CultureMech_008001 --> ing7_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing7_NH4NO3 -.-> CHEBI_63038
    ing8_NiSO4_7H2O["NiSO4・7H2O"]
    CultureMech_008001 --> ing8_NiSO4_7H2O
    CHEBI_53001["CHEBI:53001"]
    ing8_NiSO4_7H2O -.-> CHEBI_53001

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
10 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
6 CaCl2
Ca.2Cl 110.984 g/mol
10 G_PER_L CHEBI:3312
calcium dichloride
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
7 Na2HPO4
HO4P.2Na 141.958 g/mol
4.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
8 NH4NO3
H4N.NO3 80.043 g/mol
1 G_PER_L CHEBI:63038
ammonium nitrate
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
9 NiSO4・7H2O
Ni.O4S 154.756 g/mol
0.06 G_PER_L CHEBI:53001
nickel sulfate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component

Solutions

Trace element solution (see Medium [M120])

Dose: 2 G_PER_L

Cross-reference to Medium M120

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.514666Z togo-import Imported from TOGO Medium Source: JCM, ID: M145
2026-01-28T19:48:21.215580Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:35.143006+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:52.919675Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008001
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.408012+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Hydrogenobacter Thermophilus Medium" to "hydrogenobacter_thermophilus_medium" for consistent matching
2026-05-16T09:03:37.572298+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:09.056239+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:49.181137+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:59.935768+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.