blood_agar

CultureMech:008017 bacterial COMPLEX SOLID_AGAR
Original name: Blood Agar

Overview

External ontology
TOGO:M1474 — Blood Agar
Applications
  • Microbial cultivation
Notes
TOGO M1474 imports NBRC Medium 254. NBRC 254 lists 40 g Bacto Blood Agar Base (Difco) in 1 L distilled water, sterilized by autoclaving, cooled to 45-50 C, and supplemented with 50 ml sterile defibrillated blood. The source does not state pH or incubation conditions.

Composition

flowchart LR
    CultureMech_008017["blood_agar"]
    ing0_Bacto_Blood_Agar_Base__Difco_["Bacto Blood Agar Base (Difco)"]
    CultureMech_008017 --> ing0_Bacto_Blood_Agar_Base__Difco_
    ing1_Distilled_water["Distilled water"]
    CultureMech_008017 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Bacto Blood Agar Base (Difco) 40.0 G_PER_L NBRC Medium 254 lists 40 g Bacto Blood Agar Base (Difco) in 1 L distilled water; this commercial agar base is not reducible to one ChEBI molecule.
2 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 NBRC Medium 254 suspends the agar base in 1 L distilled water.

Preparation

  1. Suspend 40 g Bacto Blood Agar Base (Difco) in 1 L distilled water.
  2. Sterilize the agar base by autoclaving.
  3. Cool the autoclaved base to 45-50 C.
  4. Aseptically add 50 ml sterile defibrillated blood per liter.

Solutions

Sterile defibrillated blood

Dose: 50.0 ML_PER_L

NBRC Medium 254 cools the autoclaved agar base to 45-50 C and adds 50 ml sterile defibrillated blood per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.725058Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1474
2026-01-28T19:48:07.502994Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:53.021302Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008017
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.522117+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Blood Agar" to "blood_agar" for consistent matching
2026-06-05T07:40:33.124372+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1474_score15.py RESOLVED_TOGO_M1474_SCORE15 TOGO M1474 imports NBRC Medium 254. NBRC 254 lists 40 g Bacto Blood Agar Base (Difco) in 1 L distilled water, sterilized by autoclaving, cooled to 45-50 C, and supplemented with 50 ml sterile defibrillated blood. The source does not state pH or incubation conditions. Corrected imported blood and water amounts from g/L artifacts and marked the printed base/blood recipe as curated.