r_medium (CultureMech:008026)

Overview

External ontology

TOGO:M1482 — R medium

Applications

Microbial cultivation

pH

7.0

Notes

TOGO M1482 imports NBRC Medium 264 R medium, which lists, per liter, 10 g Peptone, 5 g Yeast extract, 5 g Malt extract, 5 g Bacto Casamino Acids (Difco), 2 g Beef extract, 2 g Glycerol, 0.05 g Tween 80, 1 g magnesium sulfate heptahydrate, 15 g Agar if needed, and final pH 7.0.

Composition

flowchart LR
    CultureMech_008026["r_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_008026 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008026 --> ing1_Yeast_extract
    ing2_Malt_extract["Malt extract"]
    CultureMech_008026 --> ing2_Malt_extract
    ing3_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
    CultureMech_008026 --> ing3_Bacto_Casamino_Acids__Difco_
    ing4_Beef_extract["Beef extract"]
    CultureMech_008026 --> ing4_Beef_extract
    ing5_Glycerol["Glycerol"]
    CultureMech_008026 --> ing5_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing5_Glycerol -.-> CHEBI_17754
    ing6_Tween_80["Tween 80"]
    CultureMech_008026 --> ing6_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing6_Tween_80 -.-> CHEBI_53426
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008026 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Distilled_water["Distilled water"]
    CultureMech_008026 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008026 --> ing9_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	Peptone	10.0 G_PER_L	MICRO:0000178 (source link unavailable) Peptone	NBRC Medium 264 lists 10.0 g/L Peptone.
2	Yeast extract	5.0 G_PER_L	FOODON:03315426 yeast extract	NBRC Medium 264 lists 5.0 g/L Yeast extract.
3	Malt extract	5.0 G_PER_L	FOODON:03301056 malt extract	NBRC Medium 264 lists 5.0 g/L Malt extract.
4	Bacto Casamino Acids (Difco)	5.0 G_PER_L	FOODON:03315719 mammalian milk protein (hydrolyzed)	NBRC Medium 264 lists 5.0 g/L Bacto Casamino Acids (Difco).
5	Beef extract	2.0 G_PER_L	FOODON:03302088 beef extract	NBRC Medium 264 lists 2.0 g/L Beef extract.
6	Glycerol C3H8O3 92.094 g/mol	2.0 G_PER_L	CHEBI:17754 glycerol	NBRC Medium 264 lists 2.0 g/L Glycerol.
7	Tween 80 (C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol	0.05 G_PER_L	CHEBI:53426 polysorbate 80	NBRC Medium 264 lists 0.05 g/L Tween 80.
8	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	1.0 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	NBRC Medium 264 lists 1.0 g/L MgSO4 x 7 H2O.
9	Distilled water H2O 18.015 g/mol	1.0 L	CHEBI:15377 water	NBRC Medium 264 lists 1.0 L Distilled water.
10	Agar (if needed)	15.0 G_PER_L	CHEBI:2509 agar	NBRC Medium 264 lists 15.0 g/L Agar if needed.

Preparation

Dissolve peptone, yeast extract, malt extract, Bacto Casamino Acids, beef extract, glycerol, Tween 80, magnesium sulfate heptahydrate, and agar if needed in 1.0 L distilled water.
Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.739813Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M1482
2026-01-28T19:48:42.263605Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:53.085512Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008026
2026-03-17T06:30:55.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.588824+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "R medium" to "r_medium" for consistent matching
2026-06-05T07:41:43.790144+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.083514+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:58:30.825944+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-09-12T00:00:00-07:00	repair_togo_m1482_m1968_r_medium_score15.py	RESOLVED_TOGO_M1482_R_MEDIUM_SCORE15	Corrected the imported distilled-water unit from g/L to L, added pH 7.0, grounded all ten disclosed components, and linked the NBRC Medium 1246 5% NaCl formulation as a salinity variant.
2026-09-12T00:00:00-07:00	repair_togo_m1482_m1968_r_medium_score15.py	LINKED_TOGO_M1968_R_MEDIUM_5_NACL_CHILD	Added or refreshed bacterial/r_medium_5_nacl.yaml as a salinity child of bacterial/r_medium.yaml.