Overview
- External ontology
- TOGO:M1483 — Glycerol-Asparagine Agar (ISP Medium No.5)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1483 Original source: NBRC - NBRC_M265 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=265
Composition
flowchart LR
CultureMech_008027["glycerol_asparagine_agar_isp_medium_no_5"]
ing0_Distilled_water["Distilled water"]
CultureMech_008027 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_K2HPO4["K2HPO4"]
CultureMech_008027 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Glycerol["Glycerol"]
CultureMech_008027 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_L_Asparagine["L-Asparagine"]
CultureMech_008027 --> ing3_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing3_L_Asparagine -.-> CHEBI_17196
ing4_Agar["Agar"]
CultureMech_008027 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008027 --> ing5_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_7H2O -.-> CHEBI_75836
ing6_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008027 --> ing6_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_4H2O -.-> CHEBI_86368
ing7_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008027 --> ing7_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_7H2O -.-> CHEBI_32312
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
10 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 4 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
1 G_PER_L |
CHEBI:17196
L-asparagine
|
Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component |
| 5 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
Solutions
Trace salts solution*
Dose: 1 G_PER_L
See mediadive_3683_Trace_salts_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.741229Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1483 |
| 2026-01-28T19:48:15.233018Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:25.378607+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:53.884783Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:53.091609Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008027 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.595154+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Glycerol-Asparagine Agar (ISP Medium No.5)" to "glycerol_asparagine_agar_isp_medium_no_5" for consistent matching |
| 2026-05-16T09:03:35.885094+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:06.550346+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.375904+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:59.138203+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |