Overview
- External ontology
- TOGO:M1492 — LB Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: https://togomedium.org/medium/M1492 Original source: NBRC - NBRC_M275 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=275
Composition
flowchart LR
CultureMech_008037["lb_medium"]
ing0_Peptone["Peptone"]
CultureMech_008037 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_008037 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008037 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Distilled_water["Distilled water"]
CultureMech_008037 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_Agar__if_needed_["Agar (if needed)"]
CultureMech_008037 --> ing4_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing4_Agar__if_needed_ -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 10 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
NBRC Medium 275 lists this generic peptone digest as a nutrient source. |
| 2 | Yeast extract | 5 G_PER_L |
FOODON:03315426
yeast extract
|
NBRC Medium 275 lists this generic yeast extract as a nutrient source. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
NBRC Medium 275 lists 5 g sodium chloride. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
NBRC Medium 275 lists 1.0 L distilled water. |
| 5 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
agar
|
NBRC Medium 275 lists 15 g agar as the optional solidifying component. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.752123Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1492 |
| 2026-01-28T19:48:15.226109Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:53.135651Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008037 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.642968+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB Medium" to "lb_medium" for consistent matching |
| 2026-06-05T07:41:10.909323+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_lb_family_score15.py | RESOLVED_LB_FAMILY_SCORE15 | TOGO M1492 mirrors NBRC Medium 275: 10 g peptone, 5 g yeast extract, 5 g NaCl, 1 L distilled water, 15 g optional agar, and pH 7.0. |