lactobacillus_medium_iii

CultureMech:008045 bacterial COMPLEX SOLID_AGAR
Original name: Lactobacillus Medium III

Overview

External ontology
TOGO:M14 — Lactobacillus Medium III
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M14 Original source: JCM - JCM_M21 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=21

Composition

flowchart LR
    CultureMech_008045["lactobacillus_medium_iii"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008045 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_008045 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_KH2PO4["KH2PO4"]
    CultureMech_008045 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_K2HPO4["K2HPO4"]
    CultureMech_008045 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_008045 --> ing4_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_7H2O -.-> CHEBI_75836
    ing5_Sodium_acetate["Sodium acetate"]
    CultureMech_008045 --> ing5_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Sodium_acetate -.-> CHEBI_32954
    ing6_Tween_80["Tween 80"]
    CultureMech_008045 --> ing6_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing6_Tween_80 -.-> CHEBI_53426
    ing7_MnSO4_xH2O["MnSO4・xH2O"]
    CultureMech_008045 --> ing7_MnSO4_xH2O
    CHEBI_86360["CHEBI:86360"]
    ing7_MnSO4_xH2O -.-> CHEBI_86360
    ing8_Ethanol["Ethanol"]
    CultureMech_008045 --> ing8_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing8_Ethanol -.-> CHEBI_16236
    ing9_DL__Mevalonic_acid["DL--Mevalonic acid"]
    CultureMech_008045 --> ing9_DL__Mevalonic_acid
    CHEBI_25351["CHEBI:25351"]
    ing9_DL__Mevalonic_acid -.-> CHEBI_25351
    ing10_Glucose["Glucose"]
    CultureMech_008045 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Agar["Agar"]
    CultureMech_008045 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509
    ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_008045 --> ing12_Yeast_extract__BD_Difco_
    ing13_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
    CultureMech_008045 --> ing13_Beef_extract__BD_Difco_
    ing14_Casein_peptone__tryptic_digest["Casein peptone, tryptic digest"]
    CultureMech_008045 --> ing14_Casein_peptone__tryptic_digest

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
4 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
10 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 Sodium acetate
C2H3O2.Na 82.034 g/mol
20 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
7 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Simple component, Organic compound, Defined component
8 MnSO4・xH2O
Mn.O4S 151.001 g/mol
7.5 G_PER_L CHEBI:86360
manganese(II) sulfate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 Ethanol
C2H6O 46.069 g/mol
40 G_PER_L CHEBI:16236
ethanol
 Role: Solvating media; Properties: Simple component, Organic compound, Defined component
10 DL--Mevalonic acid
C6H12O4 148.158 g/mol
30 G_PER_L CHEBI:25351
mevalonic acid
 Role: Growth factor; Properties: Defined component, Simple component, Organic compound
11 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
12 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
13 Yeast extract (BD-Difco) 5 G_PER_L Properties: Undefined component
14 Beef extract (BD-Difco) 2 G_PER_L Properties: Undefined component
15 Casein peptone, tryptic digest 10 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.493068Z togo-import Imported from TOGO Medium Source: JCM, ID: M14
2026-01-28T19:48:39.931323Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:53.181427Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008045
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.693671+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Lactobacillus Medium III" to "lactobacillus_medium_iii" for consistent matching
2026-06-05T07:40:33.153054+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:23.342246+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.826628+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.