Overview
- External ontology
- TOGO:M1539 — SP5 Medium
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- TOGO M1539 imports SP5 Medium from NBRC Medium 331. NBRC Medium 331 lists 9 g Bacto Casitone from Difco, 1 g Yeast extract, optional 15 g Agar, and 1 L Seawater per liter, with the final pH at 7.2. The NBRC footnote defines the seawater as filtered, aged seawater or artificial seawater from Daigo's Artificial Seawater SP.
Composition
flowchart LR
CultureMech_008086["sp5_medium"]
ing0_Bacto_Casitone__Difco_["Bacto Casitone (Difco)"]
CultureMech_008086 --> ing0_Bacto_Casitone__Difco_
ing1_Yeast_extract["Yeast extract"]
CultureMech_008086 --> ing1_Yeast_extract
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008086 --> ing2_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_needed_ -.-> CHEBI_2509
ing3_Seawater["Seawater"]
CultureMech_008086 --> ing3_Seawater
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Casitone (Difco) | 9.0 G_PER_L | TOGO M1539 / NBRC Medium 331 lists 9 g/L Bacto Casitone from Difco; this commercial casein digest is retained as an opaque complex component. | |
| 2 | Yeast extract | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M1539 / NBRC Medium 331 lists 1 g/L Yeast extract. |
| 3 | Agar (if needed) | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M1539 / NBRC Medium 331 lists 15 g/L Agar as an optional solidifying agent. |
| 4 | Seawater | 1000.0 ML_PER_L | TOGO M1539 / NBRC Medium 331 lists 1 L/L Seawater and allows either filtered, aged seawater or Daigo's Artificial Seawater SP; the exact salt composition is source-variable. |
Preparation
- Dissolve Bacto Casitone and Yeast extract in Seawater.
- Add Agar when a solid SP5 medium is needed.
- Bring SP5 Medium to pH 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.812819Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1539 |
| 2026-03-09T08:04:26.525536+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:53.423068Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008086 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:11.956095+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP5 Medium" to "sp5_medium" for consistent matching |
| 2026-05-16T09:04:31.921947+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-12T00:00:00-07:00 | repair_togo_m1539_sp5_score15.py | RESOLVED_TOGO_M1539_SP5_SCORE15 | TOGO M1539 imports SP5 Medium from NBRC Medium 331. NBRC Medium 331 lists 9 g Bacto Casitone from Difco, 1 g Yeast extract, optional 15 g Agar, and 1 L Seawater per liter, with the final pH at 7.2. The NBRC footnote defines the seawater as filtered, aged seawater or artificial seawater from Daigo's Artificial Seawater SP. Corrected the imported seawater unit, replaced the MediaDive seawater stock reference with the official NBRC source-level seawater input, grounded yeast extract and optional agar, and kept Bacto Casitone and seawater as sourced unmapped complex inputs. |