Overview
- External ontology
- TOGO:M153 — R-CW Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M153 Original source: JCM - JCM_M162 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=162
Composition
flowchart LR
CultureMech_008087["r_cw_agar"]
ing0_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008087 --> ing0_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_7H2O -.-> CHEBI_31795
ing1_KH2PO4["KH2PO4"]
CultureMech_008087 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_008087 --> ing2_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Sodium_acetate -.-> CHEBI_32954
ing3_Tween_80["Tween 80"]
CultureMech_008087 --> ing3_Tween_80
CHEBI_53426["CHEBI:53426"]
ing3_Tween_80 -.-> CHEBI_53426
ing4_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_008087 --> ing4_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing4_MnSO4_xH2O -.-> CHEBI_86360
ing5_Diammonium_hydrogen_citrate["Diammonium hydrogen citrate"]
CultureMech_008087 --> ing5_Diammonium_hydrogen_citrate
CHEBI_63076["CHEBI:63076"]
ing5_Diammonium_hydrogen_citrate -.-> CHEBI_63076
ing6_Cheese_whey["Cheese whey"]
CultureMech_008087 --> ing6_Cheese_whey
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008087 --> ing7_Yeast_extract__BD_Difco_
ing8_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_008087 --> ing8_Tryptone__BD_Difco_
ing9_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_008087 --> ing9_Trypticase_peptone__BD_BBL_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 4 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Simple component, Defined component |
| 5 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
Diammonium hydrogen citrate
C6H6O7.2H4N 226.185 g/mol
|
2 G_PER_L |
CHEBI:63076
diammonium citrate
|
Role: Nutrient source; Properties: Simple component, Defined component, Organic compound |
| 7 | Cheese whey | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 8 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 9 | Tryptone (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 10 | Trypticase peptone (BD-BBL) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.524550Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M153 |
| 2026-01-28T19:48:09.023323Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:53.427607Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008087 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.960772+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R-CW Agar" to "r_cw_agar" for consistent matching |
| 2026-06-05T07:40:33.204738+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:23.383188+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.837570+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |