marine_cytophaga_agar

CultureMech:008089 bacterial COMPLEX SOLID_AGAR
Original name: Marine Cytophaga Agar

Overview

External ontology
TOGO:M1541 — Marine Cytophaga Agar
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.4}
Notes
TOGO M1541 imports NBRC Medium 333 as Marine Cytophaga Agar. NBRC 333 lists 2 g Bacto Tryptone (Difco), 0.5 g beef extract, 0.5 g yeast extract, 0.2 g sodium acetate, optional 15 g agar, and 1 L filtered aged seawater or artificial seawater at pH 7.2-7.4. The source footnotes Daigo's Artificial Seawater SP as an artificial seawater option but does not state sterilization, incubation temperature, or other preparation steps.

Composition

flowchart LR
    CultureMech_008089["marine_cytophaga_agar"]
    ing0_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
    CultureMech_008089 --> ing0_Bacto_Tryptone__Difco_
    ing1_Beef_extract["Beef extract"]
    CultureMech_008089 --> ing1_Beef_extract
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008089 --> ing2_Yeast_extract
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_008089 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954
    ing4_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008089 --> ing4_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar__if_needed_ -.-> CHEBI_2509
    ing5_Seawater["Seawater"]
    CultureMech_008089 --> ing5_Seawater

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Bacto Tryptone (Difco) 2.0 G_PER_L NBRC Medium 333 lists 2 g/L Bacto Tryptone (Difco); this commercial peptone product is retained as an opaque complex component.
2 Beef extract 0.5 G_PER_L NBRC Medium 333 lists 0.5 g/L Beef extract; this biological extract is retained as an opaque complex component.
3 Yeast extract 0.5 G_PER_L FOODON:03315426
yeast extract
 NBRC Medium 333 lists 0.5 g/L Yeast extract.
4 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.2 G_PER_L CHEBI:32954
sodium acetate
 NBRC Medium 333 lists 0.2 g/L Sodium acetate.
5 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 NBRC Medium 333 lists 15.0 g/L Agar (if needed).
6 Seawater 1.0 L NBRC Medium 333 lists 1 L Seawater and defines it as filtered aged seawater or Daigo's Artificial Seawater SP.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.814740Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1541
2026-01-28T19:48:35.020650Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:58.173816+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:53.436738Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008089
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.969541+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Marine Cytophaga Agar" to "marine_cytophaga_agar" for consistent matching
2026-05-16T09:03:40.256294+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:12.899049+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m1541_marine_cytophaga_score15.py RESOLVED_TOGO_M1541_MARINE_CYTOPHAGA_SCORE15 TOGO M1541 imports NBRC Medium 333 as Marine Cytophaga Agar. NBRC 333 lists 2 g Bacto Tryptone (Difco), 0.5 g beef extract, 0.5 g yeast extract, 0.2 g sodium acetate, optional 15 g agar, and 1 L filtered aged seawater or artificial seawater at pH 7.2-7.4. The source footnotes Daigo's Artificial Seawater SP as an artificial seawater option but does not state sterilization, incubation temperature, or other preparation steps. Corrected the migrated Seawater* solution from 1 g/L to a 1 L medium component, added the NBRC pH range, and marked the NBRC formula as curated.