Overview
- External ontology
- TOGO:M1543 — Medium for Marine Flexibacteria
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1543 Original source: NBRC - NBRC_M335 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=335
Composition
flowchart LR
CultureMech_008091["medium_for_marine_flexibacteria"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_008091 --> ing0_Yeast_extract
ing1_KNO3["KNO3"]
CultureMech_008091 --> ing1_KNO3
CHEBI_63043["CHEBI:63043"]
ing1_KNO3 -.-> CHEBI_63043
ing2_Tris__hydroxymethyl__aminomethane["Tris (hydroxymethyl) aminomethane"]
CultureMech_008091 --> ing2_Tris__hydroxymethyl__aminomethane
ing3_Sodium_glycerophosphate["Sodium glycerophosphate"]
CultureMech_008091 --> ing3_Sodium_glycerophosphate
ing4_Agar__if_needed_["Agar (if needed)"]
CultureMech_008091 --> ing4_Agar__if_needed_
ing5_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_008091 --> ing5_Bacto_Tryptone__Difco_
ing6_Distilled_water["Distilled water"]
CultureMech_008091 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008091 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_H3BO4["H3BO4"]
CultureMech_008091 --> ing8_H3BO4
ing9_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008091 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008091 --> ing10_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_4H2O -.-> CHEBI_86368
ing11_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008091 --> ing11_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing11_CoCl2_6H2O -.-> CHEBI_35696
ing12_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008091 --> ing12_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_2H2O -.-> CHEBI_86318
ing13_ZnCl2["ZnCl2"]
CultureMech_008091 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_Sodium_tartrate["Sodium tartrate"]
CultureMech_008091 --> ing14_Sodium_tartrate
CHEBI_63017["CHEBI:63017"]
ing14_Sodium_tartrate -.-> CHEBI_63017
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
KNO3
K.NO3 101.102 g/mol
|
0.5 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Tris (hydroxymethyl) aminomethane | 1 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 4 | Sodium glycerophosphate | 0.1 G_PER_L | Role: Mineral source; Properties: Defined component, Organic compound, Simple component | |
| 5 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 6 | Bacto Tryptone (Difco) | 5 G_PER_L | Properties: Undefined component | |
| 7 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
25.2 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 | H3BO4 | 2.85 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 10 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.36 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
40.4 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 13 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
26.9 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
20.8 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 15 |
Sodium tartrate
C4H4O6.2Na 194.05 g/mol
|
1.77 G_PER_L |
CHEBI:63017
sodium L-tartrate
|
Role: Mineral source; Properties: Organic compound, Simple component, Defined component |
Solutions
Seawater**
Dose: 1 G_PER_L
See mediadive_1801_Seawater.yaml for composition
Trace element solution*
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.816903Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1543 |
| 2026-01-28T19:48:20.899759Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:34.673439+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:53.447902Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008091 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.980923+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Marine Flexibacteria" to "medium_for_marine_flexibacteria" for consistent matching |
| 2026-05-16T09:04:20.062610+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:29.153401+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |