Overview
- External ontology
- TOGO:M1544 — Cytophaga (Marine) Medium
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- TOGO M1544 imports NBRC Medium 336 as Cytophaga (Marine) Medium. NBRC 336 lists 1 g Bacto Tryptone (Difco), 1 g yeast extract, optional 15 g agar, and 1 L Seawater at pH 7.2. Its seawater footnote allows filtered aged seawater or artificial seawater using Daigo's Artificial Seawater SP. The source does not state sterilization, incubation temperature, or other preparation steps.
Composition
flowchart LR
CultureMech_008092["cytophaga_marine_medium"]
ing0_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_008092 --> ing0_Bacto_Tryptone__Difco_
ing1_Yeast_extract["Yeast extract"]
CultureMech_008092 --> ing1_Yeast_extract
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008092 --> ing2_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_needed_ -.-> CHEBI_2509
ing3_Seawater_["Seawater*"]
CultureMech_008092 --> ing3_Seawater_
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Tryptone (Difco) | 1.0 G_PER_L | NBRC Medium 336 lists 1 g/L Bacto Tryptone (Difco); this commercial peptone product is retained as an opaque complex component. | |
| 2 | Yeast extract | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M1544 / NBRC Medium 336 lists 1.0 g/L Yeast extract. |
| 3 | Agar (if needed) | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M1544 / NBRC Medium 336 lists 15.0 g/L Agar (if needed). |
| 4 | Seawater* | 1.0 L | NBRC Medium 336 lists 1 L Seawater and defines the footnote as filtered, aged seawater or artificial seawater using Daigo's Artificial Seawater SP; the seawater is retained as an opaque complex base. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.819145Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1544 |
| 2026-03-09T08:04:48.907567+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:53.453044Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008092 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:11.986440+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Cytophaga (Marine) Medium" to "cytophaga_marine_medium" for consistent matching |
| 2026-05-16T09:03:10.150151+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1544_score15.py | RESOLVED_TOGO_M1544_SCORE15 | TOGO M1544 imports NBRC Medium 336 as Cytophaga (Marine) Medium. NBRC 336 lists 1 g Bacto Tryptone (Difco), 1 g yeast extract, optional 15 g agar, and 1 L Seawater at pH 7.2. Its seawater footnote allows filtered aged seawater or artificial seawater using Daigo's Artificial Seawater SP. The source does not state sterilization, incubation temperature, or other preparation steps. Moved the imported Seawater stock solution to the main ingredient list, corrected its unit to liters, added the NBRC pH value, and marked the formula as curated. |