marine_agar_2216 (CultureMech:008095)

Overview

External ontology

TOGO:M1547 — Marine Agar 2216

Applications

Microbial cultivation

Notes

TOGO M1547 mirrors NBRC Medium 340, which lists 55.1 g Bacto Marine Agar 2216 (Difco) in 1 L distilled water and states pH unadjusted; ATCC Medium 2 confirms the same 55.1 g Marine Agar 2216 commercial product and prints the scratch Difco Marine Agar/Broth 2216 formulation with agar.

Composition

flowchart LR
    CultureMech_008095["marine_agar_2216"]
    ing0_Peptone["Peptone"]
    CultureMech_008095 --> ing0_Peptone
    ing1_Yeast_Extract["Yeast Extract"]
    CultureMech_008095 --> ing1_Yeast_Extract
    ing2_Ferric_Citrate["Ferric Citrate"]
    CultureMech_008095 --> ing2_Ferric_Citrate
    CHEBI_144421["CHEBI:144421"]
    ing2_Ferric_Citrate -.-> CHEBI_144421
    ing3_Sodium_Chloride["Sodium Chloride"]
    CultureMech_008095 --> ing3_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_Chloride -.-> CHEBI_26710
    ing4_Magnesium_Chloride["Magnesium Chloride"]
    CultureMech_008095 --> ing4_Magnesium_Chloride
    CHEBI_6636["CHEBI:6636"]
    ing4_Magnesium_Chloride -.-> CHEBI_6636
    ing5_Sodium_Sulfate["Sodium Sulfate"]
    CultureMech_008095 --> ing5_Sodium_Sulfate
    CHEBI_32149["CHEBI:32149"]
    ing5_Sodium_Sulfate -.-> CHEBI_32149
    ing6_Calcium_Chloride["Calcium Chloride"]
    CultureMech_008095 --> ing6_Calcium_Chloride
    CHEBI_3312["CHEBI:3312"]
    ing6_Calcium_Chloride -.-> CHEBI_3312
    ing7_Potassium_Chloride["Potassium Chloride"]
    CultureMech_008095 --> ing7_Potassium_Chloride
    CHEBI_32588["CHEBI:32588"]
    ing7_Potassium_Chloride -.-> CHEBI_32588
    ing8_Sodium_Bicarbonate["Sodium Bicarbonate"]
    CultureMech_008095 --> ing8_Sodium_Bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing8_Sodium_Bicarbonate -.-> CHEBI_32139
    ing9_Potassium_Bromide["Potassium Bromide"]
    CultureMech_008095 --> ing9_Potassium_Bromide
    CHEBI_32030["CHEBI:32030"]
    ing9_Potassium_Bromide -.-> CHEBI_32030
    ing10_Strontium_Chloride["Strontium Chloride"]
    CultureMech_008095 --> ing10_Strontium_Chloride
    CHEBI_36383["CHEBI:36383"]
    ing10_Strontium_Chloride -.-> CHEBI_36383
    ing11_Boric_Acid["Boric Acid"]
    CultureMech_008095 --> ing11_Boric_Acid
    CHEBI_33118["CHEBI:33118"]
    ing11_Boric_Acid -.-> CHEBI_33118
    ing12_Sodium_Silicate["Sodium Silicate"]
    CultureMech_008095 --> ing12_Sodium_Silicate
    CHEBI_60720["CHEBI:60720"]
    ing12_Sodium_Silicate -.-> CHEBI_60720
    ing13_Sodium_Fluoride["Sodium Fluoride"]
    CultureMech_008095 --> ing13_Sodium_Fluoride
    CHEBI_28741["CHEBI:28741"]
    ing13_Sodium_Fluoride -.-> CHEBI_28741
    ing14_Ammonium_Nitrate["Ammonium Nitrate"]
    CultureMech_008095 --> ing14_Ammonium_Nitrate
    CHEBI_63038["CHEBI:63038"]
    ing14_Ammonium_Nitrate -.-> CHEBI_63038
    ing15_Disodium_Phosphate["Disodium Phosphate"]
    CultureMech_008095 --> ing15_Disodium_Phosphate
    CHEBI_34683["CHEBI:34683"]
    ing15_Disodium_Phosphate -.-> CHEBI_34683
    ing16_Agar["Agar"]
    CultureMech_008095 --> ing16_Agar
    CHEBI_2509["CHEBI:2509"]
    ing16_Agar -.-> CHEBI_2509
    ing17_Distilled_water["Distilled water"]
    CultureMech_008095 --> ing17_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing17_Distilled_water -.-> CHEBI_15377

Ingredients (18)

#	Ingredient	Concentration	Ontology	Notes
1	Peptone	5.0 G_PER_L	MICRO:0000178 (source link unavailable) peptone	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
2	Yeast Extract	1.0 G_PER_L	FOODON:03315426 yeast extract	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
3	Ferric Citrate C6H5FeO7 244.944 g/mol	0.1 G_PER_L	CHEBI:144421 iron(III) citrate	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
4	Sodium Chloride Cl.Na 58.443 g/mol	19.45 G_PER_L	CHEBI:26710 sodium chloride	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
5	Magnesium Chloride 2Cl.Mg 95.211 g/mol	8.8 G_PER_L	CHEBI:6636 magnesium dichloride	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
6	Sodium Sulfate 2Na.O4S 142.043 g/mol	3.24 G_PER_L	CHEBI:32149 sodium sulfate	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
7	Calcium Chloride Ca.2Cl 110.984 g/mol	1.8 G_PER_L	CHEBI:3312 calcium dichloride	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
8	Potassium Chloride Cl.K 74.551 g/mol	0.55 G_PER_L	CHEBI:32588 potassium chloride	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
9	Sodium Bicarbonate CHO3.Na 84.006 g/mol	0.16 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
10	Potassium Bromide Br.K 119.002 g/mol	0.08 G_PER_L	CHEBI:32030 potassium bromide	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
11	Strontium Chloride 2Cl.Sr 158.526 g/mol	0.034 G_PER_L	CHEBI:36383 strontium dichloride	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
12	Boric Acid H3BO3 61.833 g/mol	0.022 G_PER_L	CHEBI:33118 boric acid	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
13	Sodium Silicate 2Na.O3Si 122.063 g/mol	0.004 G_PER_L	CHEBI:60720 sodium silicate	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
14	Sodium Fluoride F.Na 41.988 g/mol	0.0024 G_PER_L	CHEBI:28741 sodium fluoride	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
15	Ammonium Nitrate H4N.NO3 80.043 g/mol	0.0016 G_PER_L	CHEBI:63038 ammonium nitrate	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
16	Disodium Phosphate HO4P.2Na 141.958 g/mol	0.008 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation.
17	Agar	15.0 G_PER_L	CHEBI:2509 agar	ATCC Medium 2 prints 15.0 g agar in the Difco Marine Agar 2216 scratch formulation.
18	Distilled water H2O 18.015 g/mol	1000.0 ML_PER_L	CHEBI:15377 water	ATCC Medium 2 prints 1000.0 mL distilled water in the scratch formulation.

Preparation

Prepare Marine Agar 2216 from the Difco Marine Agar/Broth 2216 scratch formulation in 1000 mL distilled water, including 15.0 g agar.
Autoclave at 121 C.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Sneathiella glossodoripedis
Term
Id
NCBITaxon:1236958
Label
Sneathiella glossodoripedis JCM 23214
Genome assembly id
- GCA_000616095
- GCF_000616095.1
Strain
MKT133T; IAM 15419T; KCTC 12842T; JCM 23214T
Growth metrics
- Temperature celsius
  30.0
  Measurement conditions
  Marine Agar 2216; aerobic incubation at 30 C; source reports colonies less than 1 mm diameter after 4-5 days.
  Evidence
  Reference
  PMID:18319453
  Supports
  SUPPORT
  Snippet
  Colonies of the strain after 4-5 days incubation on marine agar 2216 at 30 degrees C were less than 1 mm in diameter.
  Explanation
  Kurahashi et al. report colony formation for strain MKT133T on Marine Agar 2216, matching the CultureMech parent medium name.
  Is max attainment
  False
Evidence
- Reference
  PMID:18319453
  Supports
  SUPPORT
  Explanation
  The species-description paper identifies MKT133T as the type strain and reports growth as colony formation on Marine Agar 2216.
- Reference
  NCBI:GCF_000616095.1
  Supports
  SUPPORT
  Explanation
  NCBI Datasets lists the genome assembly for Sneathiella glossodoripedis JCM 23214, an equivalent type-strain deposit.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.821757Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M1547
2026-01-28T19:48:23.849174Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:53.461918Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008095
2026-03-17T06:30:15.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.996162+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Marine Agar 2216" to "marine_agar_2216" for consistent matching
2026-05-05T00:00:00Z	literature_review_apply	ADDED_GROWTH_EVIDENCE	Added strain-specific Marine Agar 2216 colony-formation evidence for Sneathiella glossodoripedis MKT133T / JCM 23214.
2026-06-05T07:40:33.219863+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T00:58:02.661292+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 1 specific strain(s) named, no community signal (#142)
2026-09-10T00:00:00-07:00	repair_togo_commercial_score20.py	RESOLVED_TOGO_COMMERCIAL_SCORE20_GRAPH	TOGO M1547 mirrors NBRC Medium 340, which lists 55.1 g Bacto Marine Agar 2216 (Difco) in 1 L distilled water and states pH unadjusted; ATCC Medium 2 confirms the same 55.1 g Marine Agar 2216 commercial product and prints the scratch Difco Marine Agar/Broth 2216 formulation with agar.