Overview
- External ontology
- TOGO:M1548 — Medium for Freshwater Flexibacteria
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1548 Original source: NBRC - NBRC_M341 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=341
Composition
flowchart LR
CultureMech_008096["medium_for_freshwater_flexibacteria"]
ing0_Distilled_water["Distilled water"]
CultureMech_008096 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008096 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008096 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_Cobalamine["Cobalamine"]
CultureMech_008096 --> ing3_Cobalamine
CHEBI_28911["CHEBI:28911"]
ing3_Cobalamine -.-> CHEBI_28911
ing4_KNO3["KNO3"]
CultureMech_008096 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_Thiamine["Thiamine"]
CultureMech_008096 --> ing5_Thiamine
CHEBI_18385["CHEBI:18385"]
ing5_Thiamine -.-> CHEBI_18385
ing6_Tris__hydroxymethyl__aminomethane["Tris (hydroxymethyl) aminomethane"]
CultureMech_008096 --> ing6_Tris__hydroxymethyl__aminomethane
ing7_Sodium_glycerophosphate["Sodium glycerophosphate"]
CultureMech_008096 --> ing7_Sodium_glycerophosphate
ing8_Glucose["Glucose"]
CultureMech_008096 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Agar__if_needed_["Agar (if needed)"]
CultureMech_008096 --> ing9_Agar__if_needed_
ing10_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
CultureMech_008096 --> ing10_Bacto_Casamino_Acids__Difco_
ing11_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008096 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO4["H3BO4"]
CultureMech_008096 --> ing12_H3BO4
ing13_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008096 --> ing13_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_7H2O -.-> CHEBI_75836
ing14_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008096 --> ing14_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_4H2O -.-> CHEBI_86368
ing15_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008096 --> ing15_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2_6H2O -.-> CHEBI_35696
ing16_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008096 --> ing16_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_2H2O -.-> CHEBI_86318
ing17_ZnCl2["ZnCl2"]
CultureMech_008096 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_Sodium_tartrate["Sodium tartrate"]
CultureMech_008096 --> ing18_Sodium_tartrate
CHEBI_63017["CHEBI:63017"]
ing18_Sodium_tartrate -.-> CHEBI_63017
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Cobalamine
C62H88CoN13O14P 1329.37 g/mol
|
1 G_PER_L |
CHEBI:28911
cob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 5 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Thiamine
C12H17N4OS 265.362 g/mol
|
1 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 7 | Tris (hydroxymethyl) aminomethane | 1 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 8 | Sodium glycerophosphate | 0.1 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 10 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 11 | Bacto Casamino Acids (Difco) | 1 G_PER_L | Properties: Undefined component | |
| 12 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
25.2 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 | H3BO4 | 2.85 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 14 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.36 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
40.4 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
26.9 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
20.8 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Sodium tartrate
C4H4O6.2Na 194.05 g/mol
|
1.77 G_PER_L |
CHEBI:63017
sodium L-tartrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace element solution*
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.822481Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1548 |
| 2026-01-28T19:48:38.292379Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:04.066179+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:36.014867Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:53.469770Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008096 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.004038+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Freshwater Flexibacteria" to "medium_for_freshwater_flexibacteria" for consistent matching |
| 2026-05-16T09:04:19.986090+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:29.059504+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:20.997952+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-09-02T07:38:47.839379+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |