Overview
- External ontology
- TOGO:M1559 — SA Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1559 Original source: NBRC - NBRC_M360 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=360
Composition
flowchart LR
CultureMech_008107["sa_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008107 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008107 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008107 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008107 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008107 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_008107 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_K2HPO4["K2HPO4"]
CultureMech_008107 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_008107 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_Sodium_acetate["Sodium acetate"]
CultureMech_008107 --> ing8_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing8_Sodium_acetate -.-> CHEBI_32954
ing9_Sodium_succinate["Sodium succinate"]
CultureMech_008107 --> ing9_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing9_Sodium_succinate -.-> CHEBI_63675
ing10_Agar__if_needed_["Agar (if needed)"]
CultureMech_008107 --> ing10_Agar__if_needed_
ing11_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_008107 --> ing11_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing11_Na2S2O3_5H2O -.-> CHEBI_32150
ing12_Biotin["Biotin"]
CultureMech_008107 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008107 --> ing13_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
ing14_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008107 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_008107 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_Niacine["Niacine"]
CultureMech_008107 --> ing16_Niacine
ing17_Pyridoxal_HCl["Pyridoxal-HCl"]
CultureMech_008107 --> ing17_Pyridoxal_HCl
ing18_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008107 --> ing18_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_2H2O -.-> CHEBI_75213
ing19_H3BO3["H3BO3"]
CultureMech_008107 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008107 --> ing20_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing20_CoCl2_6H2O -.-> CHEBI_35696
ing21_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008107 --> ing21_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_6H2O -.-> CHEBI_34887
ing22_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008107 --> ing22_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_2H2O -.-> CHEBI_86318
ing23_ZnCl2["ZnCl2"]
CultureMech_008107 --> ing23_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing23_ZnCl2 -.-> CHEBI_49976
ing24_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008107 --> ing24_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing24_FeCl3_6H2O -.-> CHEBI_86254
ing25_Na2SeO3["Na2SeO3"]
CultureMech_008107 --> ing25_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing25_Na2SeO3 -.-> CHEBI_48843
ing26_MnCl2_2H2O["MnCl2·2H2O"]
CultureMech_008107 --> ing26_MnCl2_2H2O
CHEBI_131395["CHEBI:131395"]
ing26_MnCl2_2H2O -.-> CHEBI_131395
ing27_2Na_EDTA["2Na-EDTA"]
CultureMech_008107 --> ing27_2Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing27_2Na_EDTA -.-> CHEBI_64734
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
102.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 100.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 10 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
1 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 11 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 12 |
Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
30 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
50 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 | Vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 | Niacine | 50 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 18 | Pyridoxal-HCl | 10 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 19 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
20 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
10 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
10 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
2 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
5 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
MnCl2·2H2O
Cl2Mn.2H2O 161.874 g/mol
|
0.1 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
2Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
1 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Vitamin mixture*
Dose: 1 G_PER_L
See mediadive_5209_Vitamin_mixture.yaml for composition
Trace element solution**
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.834280Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1559 |
| 2026-01-28T19:48:40.583645Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:08.402283+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:40.135321Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:53.527036Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008107 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.065848+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SA Medium" to "sa_medium" for consistent matching |
| 2026-05-16T09:04:31.121375+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:44.983954+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:31.656115+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:11.210138+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |