Overview
- External ontology
- TOGO:M1572 — Ekho Lake Strains Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1572 Original source: NBRC - NBRC_M376 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=376
Composition
flowchart LR
CultureMech_008121["ekho_lake_strains_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008121 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Bacto_Agar__Difco_["Bacto Agar (Difco)"]
CultureMech_008121 --> ing1_Bacto_Agar__Difco_
ing2_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008121 --> ing2_Bacto_Yeast_Extract__Difco_
ing3_Bacto_Peptone__Difco_["Bacto Peptone (Difco)"]
CultureMech_008121 --> ing3_Bacto_Peptone__Difco_
ing4_KOH["KOH"]
CultureMech_008121 --> ing4_KOH
CHEBI_32035["CHEBI:32035"]
ing4_KOH -.-> CHEBI_32035
ing5_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008121 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008121 --> ing6_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_2H2O -.-> CHEBI_86158
ing7_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008121 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008121 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_008121 --> ing9_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing10_Biotin["Biotin"]
CultureMech_008121 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008121 --> ing11_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
ing12_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008121 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Ca_pantothenate["Ca-pantothenate"]
CultureMech_008121 --> ing13_Ca_pantothenate
CHEBI_29032["CHEBI:29032"]
ing13_Ca_pantothenate -.-> CHEBI_29032
ing14_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_008121 --> ing14_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_HCl -.-> CHEBI_30961
ing15_Folic_acid["Folic acid"]
CultureMech_008121 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_008121 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_Riboflavin["Riboflavin"]
CultureMech_008121 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinamide["Nicotinamide"]
CultureMech_008121 --> ing18_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing18_Nicotinamide -.-> CHEBI_17154
ing19_NaCl["NaCl"]
CultureMech_008121 --> ing19_NaCl
CHEBI_26710["CHEBI:26710"]
ing19_NaCl -.-> CHEBI_26710
ing20_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008121 --> ing20_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing20_MgCl2_6H2O -.-> CHEBI_86345
ing21_H3BO3["H3BO3"]
CultureMech_008121 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_KCl["KCl"]
CultureMech_008121 --> ing22_KCl
CHEBI_32588["CHEBI:32588"]
ing22_KCl -.-> CHEBI_32588
ing23_NaHCO3["NaHCO3"]
CultureMech_008121 --> ing23_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing23_NaHCO3 -.-> CHEBI_32139
ing24_Na2SO4["Na2SO4"]
CultureMech_008121 --> ing24_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing24_Na2SO4 -.-> CHEBI_32149
ing25_CaCl2["CaCl2"]
CultureMech_008121 --> ing25_CaCl2
CHEBI_3312["CHEBI:3312"]
ing25_CaCl2 -.-> CHEBI_3312
ing26_NaF["NaF"]
CultureMech_008121 --> ing26_NaF
CHEBI_28741["CHEBI:28741"]
ing26_NaF -.-> CHEBI_28741
ing27_KBr["KBr"]
CultureMech_008121 --> ing27_KBr
CHEBI_32030["CHEBI:32030"]
ing27_KBr -.-> CHEBI_32030
ing28_SrCl2["SrCl2"]
CultureMech_008121 --> ing28_SrCl2
CHEBI_36383["CHEBI:36383"]
ing28_SrCl2 -.-> CHEBI_36383
ing29_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008121 --> ing29_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing29_ZnSO4_7H2O -.-> CHEBI_32312
CultureMech_008121 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
818.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 715.0, 1.0, 1.0, 1.0, 100.0] |
| 2 | Bacto Agar (Difco) | 15 G_PER_L | Role: Solidifying component | |
| 3 | Bacto Yeast Extract (Difco) | 0.25 G_PER_L | Properties: Undefined component | |
| 4 | Bacto Peptone (Difco) | 0.25 G_PER_L | Properties: Undefined component | |
| 5 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 6 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
12.67 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
599.0 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 99.0, 500.0] |
| 10 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
4 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
10 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
10 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
10 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
20 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
4 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 | Vitamin B12 | 0.2 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
10 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
10 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 20 |
NaCl
Cl.Na 58.443 g/mol
|
23.477 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.981 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
26 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
KCl
Cl.K 74.551 g/mol
|
664 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
NaHCO3
CHO3.Na 84.006 g/mol
|
192 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.917 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.102 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
NaF
F.Na 41.988 g/mol
|
3 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
KBr
Br.K 119.002 g/mol
|
6 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
SrCl2
2Cl.Sr 158.526 g/mol
|
24 G_PER_L |
CHEBI:36383
strontium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
1095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
39.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
|
154 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
Co(NO3)2·6H2O
Co.6H2O.2NO3 291.031 g/mol
|
24.8 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
17.7 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
250 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Simple component, Organic compound |
| 36 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
Solutions
Glucose solution (2.5%, filter-sterilized)
Dose: 10 G_PER_L
Role: Carbon source; Properties: Organic compound, Defined component, Solution
Mineral salt solution* (\"Hutner/Cohen-Bazire\")
Dose: 20 G_PER_L
Vitamin solution**
Dose: 5 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Artificial sea water***
Dose: 250 G_PER_L
See mediadive_2170_Artificial_sea_water.yaml for composition
Metal salt solution 44****
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.856980Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1572 |
| 2026-01-28T19:48:24.855700Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: KOH; Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:04:41.094556+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:11.110096Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:53.642112Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008121 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.189018+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ekho Lake Strains Medium" to "ekho_lake_strains_medium" for consistent matching |
| 2026-05-16T09:03:10.244207+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:33.315078+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:30.404725+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:23.477086+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.861711+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |