Overview
- External ontology
- TOGO:M1577 — Flexiligladius Medium
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1577 imports NBRC Medium 384 as Flexiligladius Medium. NBRC 384 lists 2.5 g Bacto Heart Infusion Broth (Difco), optional 15 g agar, and 1 L distilled water; pH is unadjusted. The source does not state sterilization, incubation temperature, or other preparation steps.
Composition
flowchart LR
CultureMech_008126["flexiligladius_medium"]
ing0_Bacto_Heart_Infusion_Broth__Difco_["Bacto Heart Infusion Broth (Difco)"]
CultureMech_008126 --> ing0_Bacto_Heart_Infusion_Broth__Difco_
ing1_Agar__if_needed_["Agar (if needed)"]
CultureMech_008126 --> ing1_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing1_Agar__if_needed_ -.-> CHEBI_2509
ing2_Distilled_water["Distilled water"]
CultureMech_008126 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Heart Infusion Broth (Difco) | 2.5 G_PER_L | NBRC Medium 384 lists 2.5 g/L Bacto Heart Infusion Broth (Difco); this commercial complex product is retained as an opaque component. | |
| 2 | Agar (if needed) | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M1577 / NBRC Medium 384 lists 15.0 g/L Agar (if needed). |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
NBRC Medium 384 lists 1 L distilled water. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.870419Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1577 |
| 2026-01-28T19:48:34.064865Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:53.686649Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008126 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.234864+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Flexiligladius Medium" to "flexiligladius_medium" for consistent matching |
| 2026-06-05T07:41:00.437877+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m1577_score15.py | RESOLVED_TOGO_M1577_SCORE15 | TOGO M1577 imports NBRC Medium 384 as Flexiligladius Medium. NBRC 384 lists 2.5 g Bacto Heart Infusion Broth (Difco), optional 15 g agar, and 1 L distilled water; pH is unadjusted. The source does not state sterilization, incubation temperature, or other preparation steps. Corrected the imported water g/L artifact and marked the NBRC formula as curated. |