bifidobacterium_medium

CultureMech:008127 bacterial COMPLEX LIQUID
Original name: Bifidobacterium Medium

Overview

External ontology
TOGO:M1578 — Bifidobacterium Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1578 Original source: NBRC - NBRC_M385 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=385

Composition

flowchart LR
    CultureMech_008127["bifidobacterium_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_008127 --> ing0_Yeast_extract
    ing1_K2HPO4["K2HPO4"]
    CultureMech_008127 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Tween_80["Tween 80"]
    CultureMech_008127 --> ing2_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing2_Tween_80 -.-> CHEBI_53426
    ing3_Tap_water["Tap water"]
    CultureMech_008127 --> ing3_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Tap_water -.-> CHEBI_15377
    ing4_Glucose["Glucose"]
    CultureMech_008127 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Meat_extract["Meat extract"]
    CultureMech_008127 --> ing5_Meat_extract
    ing6_Casein_peptone__tryptic_digest["Casein peptone, tryptic digest"]
    CultureMech_008127 --> ing6_Casein_peptone__tryptic_digest
    ing7_sodium_ascorbate["sodium ascorbate"]
    CultureMech_008127 --> ing7_sodium_ascorbate
    CHEBI_113451["CHEBI:113451"]
    ing7_sodium_ascorbate -.-> CHEBI_113451
    ing8_cysteine_HCl["cysteine-HCl"]
    CultureMech_008127 --> ing8_cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing8_cysteine_HCl -.-> CHEBI_91247

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
2 K2HPO4
HO4P.2K 174.174 g/mol
3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
3 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
Role: Surfactant; Properties: Organic compound, Defined component, Simple component
4 Tap water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Undefined component
5 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Defined component, Organic compound, Simple component
6 Meat extract 5 G_PER_L Properties: Undefined component
7 Casein peptone, tryptic digest 10 G_PER_L Properties: Undefined component
8 sodium ascorbate
C6H7O6.Na 198.106 g/mol
variable VARIABLE CHEBI:113451
sodium ascorbate
Properties: Defined component, Organic compound, Solution
9 cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
variable VARIABLE CHEBI:91247
L-cysteine hydrochloride
Properties: Defined component, Solution, Organic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.871193Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1578
2026-01-28T19:48:19.048413Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: sodium ascorbate; Added default concentration for ingredient: cysteine-HCl
2026-03-13T06:19:53.691416Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008127
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:12.239750+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Bifidobacterium Medium" to "bifidobacterium_medium" for consistent matching
2026-05-16T09:03:10.279995+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:33.353852+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.878988+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.