Overview
- External ontology
- TOGO:M1591 — M1D Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1591 Original source: NBRC - NBRC_M400 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=400
Composition
flowchart LR
CultureMech_008142["m1d_medium"]
ing0_water["water"]
CultureMech_008142 --> ing0_water
CHEBI_15377["CHEBI:15377"]
ing0_water -.-> CHEBI_15377
ing1_Sucrose["Sucrose"]
CultureMech_008142 --> ing1_Sucrose
CHEBI_17992["CHEBI:17992"]
ing1_Sucrose -.-> CHEBI_17992
ing2_Fructose["Fructose"]
CultureMech_008142 --> ing2_Fructose
CHEBI_28645["CHEBI:28645"]
ing2_Fructose -.-> CHEBI_28645
ing3_Phenol_red["Phenol red"]
CultureMech_008142 --> ing3_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing3_Phenol_red -.-> CHEBI_31991
ing4_Sorbitol["Sorbitol"]
CultureMech_008142 --> ing4_Sorbitol
CHEBI_30911["CHEBI:30911"]
ing4_Sorbitol -.-> CHEBI_30911
ing5_Fetal_bovine_serum__heat_inactivated_["Fetal bovine serum (heat-inactivated)"]
CultureMech_008142 --> ing5_Fetal_bovine_serum__heat_inactivated_
ing6_Glucose["Glucose"]
CultureMech_008142 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Penicillin_G__100_000_U_ml_["Penicillin G (100,000 U/ml)"]
CultureMech_008142 --> ing7_Penicillin_G__100_000_U_ml_
ing8_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_008142 --> ing8_Bacto_Tryptone__Difco_
ing9_Bacto_Peptone__Difco_["Bacto Peptone (Difco)"]
CultureMech_008142 --> ing9_Bacto_Peptone__Difco_
ing10_Bacto_PPLO_Broth_w_o_CV__Difco_["Bacto PPLO Broth w/o CV (Difco)"]
CultureMech_008142 --> ing10_Bacto_PPLO_Broth_w_o_CV__Difco_
ing11_Schneider_s_Drosophila_medium__Invitorogen__Gibco_["Schneider's Drosophila medium (Invitorogen, Gibco)"]
CultureMech_008142 --> ing11_Schneider_s_Drosophila_medium__Invitorogen__Gibco_
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
water
H2O 18.015 g/mol
|
90 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Sucrose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17992
sucrose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 3 |
Fructose
C6H12O6 180.156 g/mol
|
0.1 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 4 |
Phenol red
C19H14O5S 354.383 g/mol
|
6 G_PER_L |
CHEBI:31991
phenol red
|
Role: pH indicator; Properties: Simple component, Organic compound, Defined component |
| 5 |
Sorbitol
C6H14O6 182.172 g/mol
|
7.5 G_PER_L |
CHEBI:30911
glucitol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 6 | Fetal bovine serum (heat-inactivated) | 50 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
0.1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 8 | Penicillin G (100,000 U/ml) | 2.5 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Simple component | |
| 9 | Bacto Tryptone (Difco) | 1 G_PER_L | Properties: Undefined component | |
| 10 | Bacto Peptone (Difco) | 0.8 G_PER_L | Properties: Undefined component | |
| 11 | Bacto PPLO Broth w/o CV (Difco) | 2 G_PER_L | Properties: Complex component, Undefined component | |
| 12 | Schneider's Drosophila medium (Invitorogen, Gibco) | 160 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.908669Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1591 |
| 2026-01-28T19:48:22.927186Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:53.876221Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008142 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.429840+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M1D Medium" to "m1d_medium" for consistent matching |
| 2026-06-05T07:41:11.771172+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |