Overview
- External ontology
- TOGO:M1621 — R Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1621 Original source: NBRC - NBRC_M821 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=821
Composition
flowchart LR
CultureMech_008176["r_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_008176 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008176 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Tween_80["Tween 80"]
CultureMech_008176 --> ing2_Tween_80
CHEBI_53426["CHEBI:53426"]
ing2_Tween_80 -.-> CHEBI_53426
ing3_Glycerol["Glycerol"]
CultureMech_008176 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_Bacto_Malt_Extract__Difco_["Bacto Malt Extract (Difco)"]
CultureMech_008176 --> ing4_Bacto_Malt_Extract__Difco_
ing5_Agar["Agar"]
CultureMech_008176 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008176 --> ing6_Bacto_Yeast_Extract__Difco_
ing7_Bacto_Peptone__Difco_["Bacto Peptone (Difco)"]
CultureMech_008176 --> ing7_Bacto_Peptone__Difco_
ing8_Bacto_Beef_Extract__Difco_["Bacto Beef Extract (Difco)"]
CultureMech_008176 --> ing8_Bacto_Beef_Extract__Difco_
ing9_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
CultureMech_008176 --> ing9_Bacto_Casamino_Acids__Difco_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
50 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Simple component, Defined component |
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 5 | Bacto Malt Extract (Difco) | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 | Bacto Yeast Extract (Difco) | 5 G_PER_L | Properties: Undefined component | |
| 8 | Bacto Peptone (Difco) | 10 G_PER_L | Properties: Undefined component | |
| 9 | Bacto Beef Extract (Difco) | 2 G_PER_L | Properties: Undefined component | |
| 10 | Bacto Casamino Acids (Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.975162Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1621 |
| 2026-01-28T19:48:12.743316Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:54.165111Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008176 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.741221+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R Agar" to "r_agar" for consistent matching |
| 2026-06-05T07:40:33.596029+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:30.578637+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:23.747436+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |