thermoproteus_neutrophilus_medium

CultureMech:008188 archaea COMPLEX LIQUID
Original name: Thermoproteus neutrophilus Medium

Overview

External ontology
TOGO:M1633 — Thermoproteus neutrophilus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1633 Original source: NBRC - NBRC_M836 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=836

Composition

flowchart LR
    CultureMech_008188["thermoproteus_neutrophilus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008188 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008188 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008188 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008188 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008188 --> ing4_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing4_Na2MoO4_2H2O -.-> CHEBI_75213
    ing5_Resazurin["Resazurin"]
    CultureMech_008188 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_NaHCO3["NaHCO3"]
    CultureMech_008188 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008188 --> ing7_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_4H2O -.-> CHEBI_86368
    ing8_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_008188 --> ing8_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_7H2O -.-> CHEBI_32312
    ing9_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008188 --> ing9_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_2H2O -.-> CHEBI_86318
    ing10__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008188 --> ing10__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing10__NH4_2SO4 -.-> CHEBI_62946
    ing11_FeCl3_6H2O["FeCl3·6H2O"]
    CultureMech_008188 --> ing11_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing11_FeCl3_6H2O -.-> CHEBI_86254
    ing12_CoSO4_7H2O["CoSO4·7H2O"]
    CultureMech_008188 --> ing12_CoSO4_7H2O
    CHEBI_91244["CHEBI:91244"]
    ing12_CoSO4_7H2O -.-> CHEBI_91244
    ing13_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_008188 --> ing13_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing13_Sulfur__powder_ -.-> CHEBI_33403
    ing14_VOSO4_xH2O["VOSO4·xH2O"]
    CultureMech_008188 --> ing14_VOSO4_xH2O
    CHEBI_87020["CHEBI:87020"]
    ing14_VOSO4_xH2O -.-> CHEBI_87020
    ing15_Na2B4O7_10H2O["Na2B4O7·10H2O"]
    CultureMech_008188 --> ing15_Na2B4O7_10H2O
    CHEBI_131366["CHEBI:131366"]
    ing15_Na2B4O7_10H2O -.-> CHEBI_131366
    ing16_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_008188 --> ing16_Bacto_Yeast_Extract__Difco_
    ing17_Na2S2O4["Na2S2O4"]
    CultureMech_008188 --> ing17_Na2S2O4
    CHEBI_66870["CHEBI:66870"]
    ing17_Na2S2O4 -.-> CHEBI_66870
    ing18_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_008188 --> ing18_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing18_Carbon_dioxide_gas -.-> CHEBI_16526
    ing19_N2["N2"]
    CultureMech_008188 --> ing19_N2
    CHEBI_17997["CHEBI:17997"]
    ing19_N2 -.-> CHEBI_17997
    ing20_Hydrogen_gas["Hydrogen gas"]
    CultureMech_008188 --> ing20_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing20_Hydrogen_gas -.-> CHEBI_18276

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
7 NaHCO3
CHO3.Na 84.006 g/mol
0.85 G_PER_L CHEBI:32139
sodium hydrogencarbonate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
8 MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
1.8 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
0.05 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
12 FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
0.02 G_PER_L CHEBI:86254
iron trichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
0.01 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 Sulfur (powder) 8 G_PER_L CHEBI:33403
elemental sulfur
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15 VOSO4·xH2O
(H2O)n.O5SV 181.021 g/mol
0.03 G_PER_L CHEBI:87020
vanadyl sulfate hydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
4.5 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 Bacto Yeast Extract (Difco) 0.2 G_PER_L Properties: Undefined component
18 Na2S2O4
2Na.O4S2 174.11 g/mol
25 G_PER_L CHEBI:66870
sodium dithionite
Properties: Defined component, Simple component, Inorganic compound
19 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
20 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: ガス
21 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
Properties: ガス

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T06:35:58.014276Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1633
2026-01-28T19:48:11.215190Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas
2026-03-13T06:19:54.278080Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008188
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:12.860593+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Thermoproteus neutrophilus Medium" to "thermoproteus_neutrophilus_medium" for consistent matching
2026-05-16T09:03:10.534786+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:33.639995+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:23.792901+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.958279+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.