Overview
- External ontology
- TOGO:M1645 — BL Agar
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1645 imports NBRC Medium 848 as BL Agar. NBRC 848 lists 58 g BL Agar from Nissui Pharmaceutical, 50 ml defibrinated horse blood, 950 ml distilled water, and no pH. NBRC 848 instructs autoclaving the base without horse blood, cooling to about 50 C, aseptically adding sterile defibrinated horse blood to 5% final, then quickly dispensing into sterile test tubes or Petri dishes.
Composition
flowchart LR
CultureMech_008201["bl_agar"]
ing0_BL_Agar_["BL Agar*"]
CultureMech_008201 --> ing0_BL_Agar_
ing1_Defibrinated_horse_blood["Defibrinated horse blood"]
CultureMech_008201 --> ing1_Defibrinated_horse_blood
ing2_Distilled_water["Distilled water"]
CultureMech_008201 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | BL Agar* | 58.0 G_PER_L | NBRC Medium 848 lists 58 g/L BL Agar and notes that the asterisk marks Nissui Pharmaceutical Co. Ltd.; this commercial BL Agar product is retained as an opaque base/solidifying component. | |
| 2 | Defibrinated horse blood | 50.0 ML_PER_L |
UBERON:0000178
blood
|
NBRC Medium 848 lists 50 ml/L sterile defibrinated horse blood added aseptically to a final 5% v/v. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
950.0 ML_PER_L |
CHEBI:15377
water
|
NBRC Medium 848 lists 950 ml/L distilled water. |
Preparation
- Autoclave the BL Agar base without horse blood.
- Cool to about 50 C.
- Aseptically add sterile defibrinated horse blood to 5% v/v final and mix.
- Quickly dispense into sterile test tubes or Petri dishes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.040809Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1645 |
| 2026-01-28T19:48:13.928216Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:23.098341+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:54.397311Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008201 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.988144+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BL Agar" to "bl_agar" for consistent matching |
| 2026-05-16T09:03:25.819919+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:51.008094+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m1645_bl_agar_score15.py | RESOLVED_TOGO_M1645_SCORE15 | TOGO M1645 imports NBRC Medium 848 as BL Agar. NBRC 848 lists 58 g BL Agar from Nissui Pharmaceutical, 50 ml defibrinated horse blood, 950 ml distilled water, and no pH. NBRC 848 instructs autoclaving the base without horse blood, cooling to about 50 C, aseptically adding sterile defibrinated horse blood to 5% final, then quickly dispensing into sterile test tubes or Petri dishes. Moved the Nissui BL Agar product out of an empty solution wrapper, corrected water and horse blood liquid units, and marked the NBRC formula as curated. |