Overview
- External ontology
- TOGO:M1649 — Pyrobaculum aerophilum Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1649 Original source: NBRC - NBRC_M852 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=852
Composition
flowchart LR
CultureMech_008205["pyrobaculum_aerophilum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008205 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_008205 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_008205 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaHCO3["NaHCO3"]
CultureMech_008205 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2·6H2O"]
CultureMech_008205 --> ing4_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing4_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing5__NH4_2Ni_SO4_2_6H2O["(NH4)2Ni(SO4)2·6H2O"]
CultureMech_008205 --> ing5__NH4_2Ni_SO4_2_6H2O
CHEBI_86149["CHEBI:86149"]
ing5__NH4_2Ni_SO4_2_6H2O -.-> CHEBI_86149
ing6_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008205 --> ing6_Bacto_Yeast_Extract__Difco_
ing7_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_008205 --> ing7_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_5H2O -.-> CHEBI_32150
ing8_H2SO4["H2SO4"]
CultureMech_008205 --> ing8_H2SO4
CHEBI_26836["CHEBI:26836"]
ing8_H2SO4 -.-> CHEBI_26836
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008205 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_008205 --> ing10_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing10_Nitrogen_gas -.-> CHEBI_17997
ing11_Oxygen_gas["Oxygen gas"]
CultureMech_008205 --> ing11_Oxygen_gas
ing12_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008205 --> ing12_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_7H2O -.-> CHEBI_31795
ing13_NaCl["NaCl"]
CultureMech_008205 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008205 --> ing14_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_2H2O -.-> CHEBI_86158
ing15_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008205 --> ing15_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing15_MgCl2_6H2O -.-> CHEBI_86345
ing16_H3BO3["H3BO3"]
CultureMech_008205 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_Na2CO3["Na2CO3"]
CultureMech_008205 --> ing17_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing17_Na2CO3 -.-> CHEBI_29377
ing18_Na2SO4["Na2SO4"]
CultureMech_008205 --> ing18_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing18_Na2SO4 -.-> CHEBI_32149
ing19_Na2HPO4["Na2HPO4"]
CultureMech_008205 --> ing19_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing19_Na2HPO4 -.-> CHEBI_34683
ing20_NaBr["NaBr"]
CultureMech_008205 --> ing20_NaBr
CHEBI_63004["CHEBI:63004"]
ing20_NaBr -.-> CHEBI_63004
ing21_SrCl2_6H2O["SrCl2·6H2O"]
CultureMech_008205 --> ing21_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing21_SrCl2_6H2O -.-> CHEBI_36385
ing22_KI["KI"]
CultureMech_008205 --> ing22_KI
CHEBI_8346["CHEBI:8346"]
ing22_KI -.-> CHEBI_8346
ing23_NaF["NaF"]
CultureMech_008205 --> ing23_NaF
CHEBI_28741["CHEBI:28741"]
ing23_NaF -.-> CHEBI_28741
ing24_Sodium_silicate["Sodium silicate"]
CultureMech_008205 --> ing24_Sodium_silicate
CHEBI_60720["CHEBI:60720"]
ing24_Sodium_silicate -.-> CHEBI_60720
ing25_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008205 --> ing25_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_2H2O -.-> CHEBI_75213
ing26_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008205 --> ing26_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing26_FeSO4_7H2O -.-> CHEBI_75836
ing27_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008205 --> ing27_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing27_NiCl2_6H2O -.-> CHEBI_34887
ing28_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008205 --> ing28_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing28_ZnSO4_7H2O -.-> CHEBI_32312
ing29_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008205 --> ing29_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing29_CuSO4_5H2O -.-> CHEBI_31440
CultureMech_008205 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
867.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 865.0, 1.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.07 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Fe(NH4)2(SO4)2·6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
2 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2Ni(SO4)2·6H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Bacto Yeast Extract (Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 8 |
Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Simple component, Inorganic compound |
| 9 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 | Oxygen gas | variable VARIABLE | Properties: ガス | |
| 13 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
10.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 7.0, 3.0] |
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
48.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 47.0, 1.0] |
| 15 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.0, 0.1] |
| 16 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
18 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
52.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 52.0, 0.01] |
| 18 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.1 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.13 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
8.1 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
SrCl2·6H2O
Cl2Sr.6H2O 266.616 g/mol
|
72 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
KI
I.K 166.002 g/mol
|
0.05 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
NaF
F.Na 41.988 g/mol
|
2.4 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Sodium silicate
2Na.O3Si 122.063 g/mol
|
0.4 G_PER_L |
CHEBI:60720
sodium silicate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.4 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
KAl(SO4)2·12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
2 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 35 |
MnSO4·2H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.5 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 36 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.4 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 37 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
Marine medium/synthetic seawater mix solution*
Dose: 125 G_PER_L
See mediadive_3882_Marine_medium_Synthetic_seawater_mix_solution.yaml for composition
Trace elements**
Dose: 10 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.050730Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1649 |
| 2026-01-28T19:48:39.386532Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas; Added default concentration for ingredient: KOH |
| 2026-03-09T08:05:06.204263+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:37.983603Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:54.450968Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008205 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.043078+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pyrobaculum aerophilum Medium" to "pyrobaculum_aerophilum_medium" for consistent matching |
| 2026-05-16T09:03:10.631561+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:33.740051+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:23.872134+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.993321+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |